ANB1 / YJR047C Overview
- Standard Name
- ANB1 1
- Systematic Name
- YJR047C
- SGD ID
- SGD:S000003808
- Aliases
- eIF5A , eIF-5A 4 , HYP1 8 , TIF51B 4
- Feature Type
- ORF , Verified
- Description
- Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant 2 3 4 5 6
- Name Description
- ANaeroBically induced 1
- Paralog
- HYP2 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Anb1p is 157 amino acids long, extremely long-lived, moderate in abundance; contains a nucleic acid-binding OB-fold; hypusinated at K51, sumoylated at 8 lysines, phosphorylated on 5 residues
- Length (a.a.)
- 157
- Mol. Weight (Da)
- 17120.5
- Isoelectric Point
- 4.57
- Median Abundance (molecules/cell)
- 7323 +/- 6877
- Half-life (hr)
- 77.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all ANB1 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ribosome binding protein involved in positive regulation of translational elongation and termination; localizes to cytosolic ribosome
View computational annotations
Molecular Function
- Manually Curated
- enables ribosome binding (ISA)
- enables RNA binding (ISA)
- enables translation elongation factor activity (ISA)
Biological Process
- Manually Curated
- involved in positive regulation of translational elongation (IGI)
- involved in positive regulation of translational termination (ISA)
- involved in translational frameshifting (IGI)
Cellular Component
- Manually Curated
- located in cytosolic ribosome (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is unable to grow under anaerobic conditions and displays decreased competitive fitness on minimal medium; null mutant displays greatly decreased filamentous growth and is unable to undergo invasive growth; null mutant displays decreased calcium ion accumulation
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Anb1p interacts physically with proteins involved in mitotic cell cycle and tRNA aminoacylation; ANB1 interacts genetically with genes involved in transcription and regulation of translation; the anb1 null mutant is viable, the null mutant of paralog hyp2 is inviable in the S288C and W303 backgrounds but viable in others, the anb1 hyp2 double mutant is inviable
101 total interactions for 90 unique genes
Physical Interactions
- Affinity Capture-MS: 38
- Affinity Capture-RNA: 1
- Co-fractionation: 1
- Protein-RNA: 1
- Reconstituted Complex: 6
- Two-hybrid: 2
Genetic Interactions
- Dosage Rescue: 3
- Negative Genetic: 34
- Phenotypic Enhancement: 1
- Positive Genetic: 6
- Synthetic Growth Defect: 4
- Synthetic Lethality: 1
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 16
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2009-08-31
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.