BAS1 / YKR099W Overview
- Standard Name
- BAS1 1
- Systematic Name
- YKR099W
- SGD ID
- SGD:S000001807
- Feature Type
- ORF , Verified
- Description
- Myb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; controls cellular ATP levels; also involved in regulation of meiotic recombination at specific genes 2 3 4 5
- Name Description
- BASal 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Bas1p is 811 amino acids long, short-lived, low in abundance; contains 2 SANT/Myb domains; acetylated on K690, phosphorylated on 20 residues
- Length (a.a.)
- 811
- Mol. Weight (Da)
- 89607.4
- Isoelectric Point
- 8.42
- Median Abundance (molecules/cell)
- 1754 +/- 711
- Half-life (hr)
- 5.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all BAS1 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding RNA polymerase II transcription activator; localizes to nucleus
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in positive regulation of transcription by RNA polymerase II (IDA, IMP, IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has abnormal vacuolar morphology, decreased transposition, increased replicative lifespan, is resistant to oxidative stress, sensitive to desiccation, impaired in endocytosis, and auxotrophic for adenine; null mutant also shows increased invasive growth; heterozygous diploid null is haploinsufficient; overexpression slows growth
Classical Genetics
- null
- cell cycle progression in G1 phase: delayed
- chemical compound accumulation: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- endocytosis: decreased
- haploinsufficient
- oxidative stress resistance: increased
- protein/peptide accumulation: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: decreased
- transposable element transposition: decreased
- utilization of carbon source: decreased rate
- vacuolar morphology: abnormal
- viable
- overexpression
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Bas1p interacts physically with proteins involved in regulation of cell cycle; BAS1 interacts genetically with genes involved in transcription
379 total interactions for 295 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 2
- Co-localization: 1
- Co-purification: 1
- Protein-RNA: 1
- Two-hybrid: 11
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 1
- Negative Genetic: 247
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 1
- Positive Genetic: 89
- Synthetic Growth Defect: 4
- Synthetic Lethality: 3
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- BAS1 encodes a transcription factor of the Myb family. Bas1p acts in concert with Pho2p to activate transcription of purine biosynthetic genes. Its regulatory targets include most genes of the AMP biosynthetic pathway, histidine and glutamine biosynthetic genes, and genes involved in one-carbon compound metabolism. Bas1p also activates serine biosynthesis under anaerobic conditions and is required for anaerobic growth. Bas1p is thought to bind to promoters first and then recruit Pho2p. The interaction between Bas1p and Pho2p is enhanced in the absence of adenine, via Pho2p sensing of the adenine pathway intermediates AICAR (5'-phosphoribosyl-5-amino-4-imidazole carboxamide) and SAICAR (succinyl-AICAR). Interaction with Pho2p unmasks the Bas1p activation domain.
- Regulators
- 0
- Targets
- 133
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-07-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.