RLM1 / YPL089C Overview
- Standard Name
- RLM1 1
- Systematic Name
- YPL089C
- SGD ID
- SGD:S000006010
- Feature Type
- ORF , Verified
- Description
- MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication 1 2 3 4 5 6
- Name Description
- Resistance to Lethality of MKK1P386 overexpression 1
- Paralog
- SMP1 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- RLM1 has a paralog, SMP1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 676
- Mol. Weight (Da)
- 73496.0
- Isoelectric Point
- 9.84
- Median Abundance (molecules/cell)
- 710 +/- 377
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RLM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding RNA polymerase II transcription factor involved in signal transduction; activates gene expression in response to cell wall stress and heat; localizes to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables DNA binding, bending (IDA)
- enables DNA-binding transcription factor activity, RNA polymerase II-specific (IDA)
- enables sequence-specific DNA binding (IDA)
Biological Process
- Manually Curated
- involved in cellular response to heat (IMP)
- involved in cellular response to stress (IMP, IDA)
- involved in positive regulation of transcription by RNA polymerase II (IDA, IMP)
- involved in regulation of flocculation (IMP)
- involved in regulation of G1 to G0 transition (IMP)
- involved in signal transduction (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- chemical compound accumulation: increased
- nuclear position: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- resistance to enzymatic treatment: decreased
- RNA accumulation: decreased
- toxin resistance: increased
- transposable element transposition: increased
- null
- auxotrophy
- competitive fitness: decreased
- competitive fitness: increased
- killer toxin resistance: increased
- metal resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: increased
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The rlm1 null mutant is viable; the null mutant of paralog smp1 is viable; the rlm1 smp1 double mutant has not been annotated for phenotype.
204 total interactions for 133 unique genes
Physical Interactions
- Affinity Capture-MS: 15
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 2
- Biochemical Activity: 2
- Co-localization: 4
- PCA: 2
- Protein-RNA: 1
- Reconstituted Complex: 1
- Two-hybrid: 8
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 4
- Negative Genetic: 115
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 7
- Positive Genetic: 10
- Synthetic Growth Defect: 8
- Synthetic Lethality: 7
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- RLM1 encodes a transcription factor of the MADS family that is primarily involved in the cell wall integrity pathway. Like other MADS family members, Rlm1p bends DNA as it binds; however, the extent of bending is less than is seen for some other family members. Rlm1p is constitutively located in the nucleus. It is phosphorylated by the Slt2p kinase upon cell wall stress, and phosphorylated Rlm1p activates transcription of genes involved in cell wall biosynthesis and maintenance. Rlm1p is also involved in transcription of genes required for tolerance of low pH stress. Under conditions of high osmolarity, Rlm1p is stimulated by the HOG pathway to activate transcription of Slt2p, creating a positive feedback loop. RLM1 has a paralog, SMP1, that encodes a transcription factor involved in osmotic stress response.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.