TOR2 / YKL203C Overview
- Standard Name
- TOR2 1
- Systematic Name
- YKL203C
- SGD ID
- SGD:S000001686
- Aliases
- DRR2 44
- Feature Type
- ORF , Verified
- Description
- PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates homeostasis of plasma membrane (PM) tension and cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; PM retention is dependent on Avo3p; malonyl-CoA is a direct ATP-competitive inhibitor of TORC1; TOR2 has a paralog, TOR1, that arose from the whole genome duplication 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
- Name Description
- Target Of Rapamycin 1
- Paralog
- TOR1 12
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- TOR2 has a paralog, TOR1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 2474
- Mol. Weight (Da)
- 281568.7
- Isoelectric Point
- 7.54
- Median Abundance (molecules/cell)
- 1773 +/- 628
- Half-life (hr)
- 9.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TOR2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine kinase that is a subunit of the TORC1 and TORC2 complexes; involved in TOR signaling and the regulation of various biological processes including cell growth, autophagy, endocytosis, and the cell cycle; involved in ribosome biogenesis as well as cytoskeleton organization and polarity; localizes to the cytoplasm, plasma membrane, and vacuole membrane
View computational annotations
Molecular Function
- Manually Curated
- enables protein binding (IPI)
- enables protein serine/threonine kinase activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in cellular response to nitrogen starvation (IGI)
- involved in cytoskeleton organization (IMP)
- involved in establishment or maintenance of actin cytoskeleton polarity (IMP)
- involved in negative regulation of autophagy (IGI)
- involved in positive regulation of endocytosis (IMP)
- involved in positive regulation of Rho protein signal transduction (IGI, IMP)
- involved in regulation of snRNA pseudouridine synthesis (IMP)
- involved in ribosome biogenesis (IMP)
- involved in TOR signaling (IMP)
Cellular Component
- Manually Curated
- located in fungal-type vacuole membrane (IDA)
- located in mitochondrion (HDA)
- located in plasma membrane (IDA)
- part of TORC1 complex (IPI)
- part of TORC2 complex (IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; heterozygous diploid null mutants are haploinsufficient and sensitive to TOR inhibitor everolimus; various alleles confer resistance to TOR inhibitor sirolimus (rapamycin); some conditional mutants are sensitive to microtubule-destabilizing agent benomyl
Classical Genetics
- null
- unspecified
- conditional
- reduction of function
- null
- overexpression
- reduction of function
- unspecified
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The tor2 null mutant is inviable; the null mutant of paralog tor1 is viable; the tor2 tor1 double mutant displays negative genetic interactions.
750 total interactions for 501 unique genes
Physical Interactions
- Affinity Capture-MS: 29
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 51
- Biochemical Activity: 17
- Co-crystal Structure: 1
- Co-fractionation: 48
- Co-localization: 2
- Co-purification: 7
- PCA: 37
- Reconstituted Complex: 8
- Two-hybrid: 7
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 28
- Negative Genetic: 372
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 5
- Positive Genetic: 75
- Synthetic Growth Defect: 12
- Synthetic Lethality: 2
- Synthetic Rescue: 34
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-10-31
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.