DNM1 / YLL001W Overview
- Standard Name
- DNM1 1
- Systematic Name
- YLL001W
- SGD ID
- SGD:S000003924
- Aliases
- DRP1 8
- Feature Type
- ORF , Verified
- Description
- Dynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and inheritance; self assembles on the cytoplasmic face of mitochondrial tubules at sites where division will occur; participates in endocytosis and regulates peroxisome fission along with Vps1p; mutants in the human ortholog DNM1L, which mediates mitochondrial fission, peroxisomal division, autophagy, and mitophagy, are associated with slowly progressive infantile encephalopathy 1 2 3 4 5 6 7
- Name Description
- DyNaMin-related 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- DNM1/YLL001W is on the left arm of chromosome XII between the centromere and RTT109 histone acetyltransferase; coding sequence is 2274 nucleotides long with 13 SNPs, one of which causes an Asn/Lys polymorphism at residue 114
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Dnm1p is 757 amino acids long, of average half-life, low-to-moderate in abundance; contains 6 dynamin domains, phosphorylated on 16 residues
- Length (a.a.)
- 757
- Mol. Weight (Da)
- 84956.7
- Isoelectric Point
- 5.16
- Median Abundance (molecules/cell)
- 7723 +/- 4585
- Half-life (hr)
- 10.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DNM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- GTPase involved in localization, organization, fission, and inheritance of mitochondria; also involved in macroautophagy, and peroxisome organization and fission; localizes to cytosol, peroxisome, and mitochondrial outer membrane
View computational annotations
Molecular Function
- Manually Curated
- enables GTPase activity (IDA, IMP)
- enables identical protein binding (IDA)
Biological Process
- Manually Curated
- involved in heme transport (IMP)
- acts upstream of or within intracellular distribution of mitochondria (IGI)
- involved in macroautophagy (IMP)
- acts upstream of or within membrane bending (IDA)
- involved in mitochondrial fission (IMP, IGI)
- involved in mitochondrion inheritance (IGI)
- involved in mitochondrion organization (IMP)
- involved in peroxisome fission (IGI)
- involved in peroxisome organization (IMP, IGI)
Cellular Component
- Manually Curated
- located in cytosol (IDA)
- located in mitochondrial outer membrane (IDA)
- located in mitochondrion (HDA, IDA)
- located in peroxisome (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant exhibits reduced autophagy and apoptosis, and increased chronological lifespan; null mutant has aberrant mitochondrial and peroxisomal morphology; in large-scale studies, null mutant shows reduced competitive fitness in several media, reduced desiccation resistance and innate thermotolerance, lower spore viability, and altered resistance to a variety of chemicals
Classical Genetics
- null
- apoptosis: decreased
- chemical compound accumulation: decreased
- endomembrane system morphology: abnormal
- lifespan: increased
- mitochondrial genome maintenance: decreased
- mitochondrial morphology: abnormal
- mitophagy: decreased
- peroxisomal morphology: abnormal
- petite
- protein/peptide accumulation: increased
- resistance to chemicals: increased
- respiratory growth: decreased
- respiratory metabolism: decreased
- small molecule transport: increased
- survival rate in stationary phase: increased
- toxin resistance: increased
- viability: decreased
- viability: increased
- viable
- reduction of function
- dominant negative
- overexpression
- null
- chemical compound accumulation: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- innate thermotolerance: decreased
- mitochondrial morphology: abnormal
- mitophagy: absent
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- viable
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast DNM1 is homologous to human DNM1L, and has been used to study mutations found in patients with infantile slowly progressive encephalopathy due to defective mitochondrial and peroxisomal fission 1
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Dnm1p interacts physically with proteins involved in organelle fission; DNM1 interacts genetically with genes involved in mitochondrion organization
445 total interactions for 254 unique genes
Physical Interactions
- Affinity Capture-MS: 33
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 19
- Co-fractionation: 1
- Co-localization: 4
- Co-purification: 2
- FRET: 2
- PCA: 8
- Proximity Label-MS: 1
- Reconstituted Complex: 3
- Two-hybrid: 21
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 221
- Phenotypic Enhancement: 47
- Phenotypic Suppression: 20
- Positive Genetic: 29
- Synthetic Growth Defect: 15
- Synthetic Lethality: 3
- Synthetic Rescue: 11
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- DNM1 promoter is bound by Med4p and Tfc7p in response to heat
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-03
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.