Complex: DNA-directed RNA polymerase II complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
- Curated by
- IntAct
- ComplexAc
- CPX-2662
- Systematic Name
- RPB2:RPB3:RPB4:RPB5:RPB7:RPB8:RPB9:RPB10:RPB11:RPC10:RPO21:RPO26
- SGD ID
- SGD:S000218120
- Aliases
- Pol II ; RNA polymerase 2 complex ; RNAPII complex ; RNApII complex
- Description
- Catalyzes the transcription of RNA from a DNA template by acting as a nucleotidyl transferase that polymerizes ribonucleotides at the 3' end of an RNA transcript. Synthesizes precursors of mRNAs, and most snRNA and microRNAs. During a transcription cycle, Pol II, general transcription factors and the mediator complex (CPX-3226) assemble as the preinitiation complex (PIC) at the promoter. 11-15 base pairs of DNA surrounding the transcription start site are melted and the single-stranded DNA template strand of the promoter is positioned deeply within the central active site cleft of Pol II to form the open complex. After synthesis of about 30 bases of RNA, Pol II releases its contacts with the core promoter and the rest of the transcription machinery (promoter clearance) and enters the stage of transcription elongation in which it moves on the template as the transcript elongates. Pol II appears to oscillate between inactive and active conformations at each step of nucleotide addition.
Complex Diagram
This diagram displays the protein subunits (blue) of the complex and how they interact with each other. Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red) are also shown. If a participant appears in the diagram with no lines connected to it, then its specific interactions with other participants are currently unknown.
PDB Structure
When available from the Protein Data Bank (PDB), the physical structure of the macromolecular complex is displayed. Click on the image or link for further information about the physical structure.
Subunits
This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. A brief description is also provided for each participant in the table.
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| Subunit | Description | Stoichiometry |
|---|
Gene Ontology
Gene Ontology (GO) terms that describe the function of a complex, the biological process in which it participates, or its cellular location. Click on a term for its definition and other objects annotated to that term.
- Molecular Function
-
- enables DNA-directed 5'-3' RNA polymerase activity (IDA)
- enables RNA polymerase II transcription regulatory region sequence-specific DNA binding (IPI)
- enables single-stranded RNA binding (IPI)
- Biological Process
-
- involved in transcription elongation by RNA polymerase II (IDA)
- involved in transcription initiation at RNA polymerase II promoter (IDA)
Shared Annotations
This diagram displays Gene Ontology terms (green) and subunits (blue) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). The shared GO terms or subunits can be visualized separately using the radio button at the bottom of the graph. Note: No diagram is shown ("No shared annotations") if there are less than 2 shared annotations (either GO terms or subunits of other complexes) between this complex and any other complexes.
Resources
IntAct | ModelArchive | PDB:1I3Q | PDB:1I50 | PDB:1I6H | PDB:1K83 | PDB:1R9S | PDB:1R9T | PDB:1SFO | PDB:1TWA | PDB:1TWC ... Show all | PDB:1TWF | PDB:1TWG | PDB:1TWH | PDB:1Y14 | PDB:2B63 | PDB:2B8K | PDB:2E2H | PDB:2NVQ | PDB:2NVT | PDB:2NVX | PDB:2NVY | PDB:2NVZ | PDB:2YU9 | PDB:3GTG | PDB:3GTJ | PDB:3GTK | PDB:3GTL | PDB:3GTO | PDB:3GTP | PDB:3M3Y | PDB:3M4O | PDB:3RZD | PDB:3RZO | PDB:3S14 | PDB:3S15 | PDB:3S16 | PDB:3S17 | PDB:3S1M | PDB:3S1N | PDB:3S1Q | PDB:3S1R | PDB:3S2D | PDB:3S2H | PDB:4X67 | PDB:4X6A | PDB:4Y52 | PDB:4Y7N | PDB:5C4J Show fewer