IRA1 / YBR140C Overview
- Standard Name
- IRA1 1
- Systematic Name
- YBR140C
- SGD ID
- SGD:S000000344
- Aliases
- GLC1 22 , PPD1 23
- Feature Type
- ORF , Verified
- Description
- GTPase-activating protein; negatively regulates RAS by converting it from GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; mutations cause catalase T deficiency, defective glycogen synthesis and defective trehalose accumulation; IRA1 has a paralog, IRA2, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis 1 2 3 4 5 6
- Name Description
- Inhibitory Regulator of the RAS-cAMP pathway 1
- Paralog
- IRA2 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- IRA1 has a paralog, IRA2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 3092
- Mol. Weight (Da)
- 350948.8
- Isoelectric Point
- 6.54
- Median Abundance (molecules/cell)
- 1512 +/- 866
- Half-life (hr)
- 6.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all IRA1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- GTPase activator with role in adenylate cyclase-inhibiting GPCR signaling pathway; involved in regulation of adenylate cyclase activity and negative regulation of Ras protein signal transduction; localizes to membrane and mitochondrion in high-throughput studies
View computational annotations
Molecular Function
- Manually Curated
- enables GTPase activator activity (IMP)
Biological Process
- Manually Curated
Cellular Component
- Manually Curated
- located in membrane (IMP)
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant accumulates cAMP and shows decreased innate thermotolerance, sensitivity to starvation, and inability to sporulate in diploids; reduction of function leads to decreased glycogen and trehalose accumulation
Classical Genetics
- unspecified
- reduction of function
- null
- reduction of function
- null
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast IRA1 is homologous to human BANF1 and NF1, and has been used to study neurofibromatosis
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The ira1 null mutant is inviable; the null mutant of paralog ira2 is viable; the ira1 ira2 double mutant displays a synthetic growth defect.
102 total interactions for 72 unique genes
Physical Interactions
- Affinity Capture-MS: 22
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 2
- Co-fractionation: 1
- PCA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 11
- Negative Genetic: 13
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 3
- Positive Genetic: 3
- Synthetic Growth Defect: 10
- Synthetic Lethality: 6
- Synthetic Rescue: 17
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-02-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.