CDC28 / YBR160W Overview
- Standard Name
- CDC28 1 2
- Systematic Name
- YBR160W
- SGD ID
- SGD:S000000364
- Aliases
- CDK1 , HSL5 , SRM5 63
- Feature Type
- ORF , Verified
- Description
- Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant 3 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
- Name Description
- Cell Division Cycle 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cdc28p is 298 amino acids long, of average half-life, low abundance; phosphorylated on 9 residues
- Length (a.a.)
- 298
- Mol. Weight (Da)
- 34064.8
- Isoelectric Point
- 8.55
- Median Abundance (molecules/cell)
- 6670 +/- 1539
- Half-life (hr)
- 10.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CDC28 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine kinase subunit of multiple cyclin-dependent kinase (CDK) complexes, which regulate the mitotic and meiotic cell cycles as well as many other cellular processes; CDK complexes function at various locations throughout the cell
View computational annotations
Molecular Function
- Manually Curated
- enables cyclin-dependent protein serine/threonine kinase activity (IDA)
- enables histone binding (IDA)
- enables protein kinase activity (HDA)
- enables protein serine/threonine kinase activity (IMP, IDA)
- enables RNA polymerase II complex binding (IDA)
Biological Process
- Manually Curated
- involved in 7-methylguanosine mRNA capping (IMP)
- involved in DNA double-strand break processing (IMP)
- involved in double-strand break repair via nonhomologous end joining (IMP)
- involved in G1/S transition of mitotic cell cycle (IDA)
- involved in G2/M transition of mitotic cell cycle (IDA)
- involved in meiotic DNA double-strand break processing (IGI)
- involved in mitotic cell cycle (IMP)
- involved in mitotic sister chromatid biorientation (IGI)
- involved in mitotic spindle assembly (IGI)
- involved in negative regulation of DNA-templated transcription (IMP, IDA)
- involved in negative regulation of double-strand break repair via nonhomologous end joining (IMP)
- involved in negative regulation of meiotic cell cycle (IMP)
- involved in negative regulation of mitotic actomyosin contractile ring assembly (IMP)
- involved in negative regulation of mitotic cell cycle (IDA)
- involved in negative regulation of sister chromatid cohesion (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP)
- involved in positive regulation of DNA-templated transcription (IGI, IDA)
- involved in positive regulation of double-strand break repair via homologous recombination (IMP)
- involved in positive regulation of glycogen catabolic process (IMP)
- involved in positive regulation of meiotic cell cycle (IMP, IDA)
- involved in positive regulation of mitotic cell cycle (IMP)
- involved in positive regulation of mitotic DNA damage checkpoint (IMP)
- involved in positive regulation of mitotic spindle pole body separation (IMP, IGI)
- involved in positive regulation of nuclear cell cycle DNA replication (IMP, IDA)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in positive regulation of trehalose catabolic process (IMP, IGI)
- involved in positive regulation of triglyceride catabolic process (IMP, IGI)
- involved in post-Golgi vesicle-mediated transport (IMP)
- involved in protein catabolic process (IMP, IGI)
- involved in protein localization to nuclear periphery (IMP)
- involved in protein localization to nucleus (IMP)
- involved in protein localization to spindle microtubule (IMP)
- involved in regulation of budding cell apical bud growth (IMP, IGI)
- involved in regulation of filamentous growth (IMP)
- involved in regulation of protein localization (IMP)
- involved in regulation of protein localization to chromatin (IDA)
- involved in regulation of spindle assembly (IMP)
- involved in regulation of telomere maintenance via telomerase (IGI)
- involved in synaptonemal complex assembly (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA)
- part of cyclin-dependent protein kinase holoenzyme complex (IDA)
- located in cytoplasm (IDA)
- located in cytoplasmic stress granule (HDA)
- located in endoplasmic reticulum (IDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutants display allele specific cell cycle arrest as either large, unbudded, conjugation-competent cells with a single, undivided nucleus and pear-shaped morphology at START in the G1 phase or as abnormally large, elongated cells with undivided nuclei at the G2/M phase transition; reduction-of-function mutant has a decreased rate of growth and buds in a bipolar or unipolar manner; conditional mutants are benomyl sensitive, have an elevated rate of plasmid and chromosome loss, and accumulate trigylcerides; homozygous diploid mutants have a sporulation defect
Classical Genetics
- reduction of function
- conditional
- actin cytoskeleton morphology: abnormal
- bipolar budding pattern: increased
- budding: delayed
- budding: increased
- cell cycle passage through START: arrested
- cell cycle passage through START: normal
- cell cycle progression in G1 phase: arrested
- cell cycle progression through the G2/M phase transition: arrested
- cell cycle progression: arrested
- cell shape: abnormal
- cell size: increased
- cellular morphology: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chitin deposition: increased
- chromosome/plasmid maintenance: decreased
- growth in exponential phase: arrested
- heat sensitivity: increased
- mitochondrial rho- mutation frequency: decreased
- protein activity: decreased
- protein activity: increased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- sporulation: decreased
- viability: decreased
- overexpression
- dominant negative
- unspecified
- null
- reduction of function
- overexpression
- repressible
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cdc28p interacts physically with proteins involved in mitosis; CDC28 interacts genetically with genes involved in transcription
2660 total interactions for 1574 unique genes
Physical Interactions
- Affinity Capture-MS: 327
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 75
- Biochemical Activity: 539
- Co-localization: 6
- Co-purification: 10
- PCA: 47
- Protein-peptide: 2
- Proximity Label-MS: 4
- Reconstituted Complex: 50
- Two-hybrid: 36
Genetic Interactions
- Dosage Growth Defect: 6
- Dosage Lethality: 397
- Dosage Rescue: 48
- Negative Genetic: 654
- Phenotypic Enhancement: 63
- Phenotypic Suppression: 30
- Positive Genetic: 113
- Synthetic Growth Defect: 168
- Synthetic Lethality: 40
- Synthetic Rescue: 42
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-08-09
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.