VAC8 / YEL013W Overview
- Standard Name
- VAC8
- Systematic Name
- YEL013W
- SGD ID
- SGD:S000000739
- Aliases
- YEB3 5
- Feature Type
- ORF , Verified
- Description
- Vacuole-specific Myo2p receptor; Myo2p-Vac17p-Vac8p transport complex subunit required for vacuolar inheritance; mediates anchoring of phosphatidylinositol 3-kinase complex I (PIK3C3-C1) to autophagosome; required with Atg13p for the vesicle closure step of the cytoplasm-to-vacuole (CVT) pathway, for homotypic vacuole-vacuole fusion and for nucleus-vacuole junction formation with Nvj1p; contains 11 armadillo (ARM) repeats; myristoylated, palmitoylated, and phosphorylated 1 2 3 4 5 6 7
- Name Description
- VACuole related
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 578
- Mol. Weight (Da)
- 63187.5
- Isoelectric Point
- 4.76
- Median Abundance (molecules/cell)
- 9669 +/- 2517
- Half-life (hr)
- 10.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all VAC8 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the MYO2-VAC17-VAC8 transport complex and NVJ1-VAC8 nucleus-vacuole junction complex; acts as protein-membrane adaptor; involved in protein localization to membranes and assembly of autophagosome, nucleus-vacuole junction, Cvt vesicle; also involved in vacuole fusion and inheritance; localized to vacuole membranes
View computational annotations
Molecular Function
- Manually Curated
- enables protein-membrane adaptor activity (IMP, IPI)
Biological Process
- Manually Curated
- involved in autophagosome assembly (IMP)
- involved in autophagy (IDA)
- involved in Cvt vesicle assembly (IMP)
- acts upstream of or within establishment of organelle localization (IMP)
- involved in macroautophagy (IMP)
- involved in nucleus-vacuole junction assembly (IMP)
- involved in pexophagy (IDA)
- involved in piecemeal microautophagy of the nucleus (IMP)
- involved in protein localization to membrane raft (IMP)
- acts upstream of or within protein localization to phagophore assembly site (IMP)
- acts upstream of or within protein-containing complex localization (IMP)
- involved in ribophagy (IDA)
- involved in vacuole fusion, non-autophagic (IMP)
- involved in vacuole inheritance (IMP)
Cellular Component
- Manually Curated
- located in cytosol (HDA)
- located in fungal-type vacuole membrane (IDA, HDA)
- located in membrane (IDA)
- located in membrane raft (IDA)
- part of Myo2p-Vac17p-Vac8p transport complex (IPI, IDA)
- located in nuclear membrane (IDA)
- located in nucleus-vacuole junction (IDA, IPI)
- located in phagophore assembly site (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- autophagy: decreased
- chemical compound accumulation: increased
- metal resistance: increased
- prion formation: increased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- replicative lifespan: decreased
- resistance to chemicals: decreased
- vacuolar distribution: abnormal
- vacuolar morphology: abnormal
- null
- acquired thermotolerance: decreased
- auxotrophy
- cell cycle progression in G1 phase: increased duration
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- colony appearance: abnormal
- competitive fitness: decreased
- endocytosis: decreased
- haploproficient
- heat sensitivity: increased
- oxidative stress resistance: increased
- protein activity: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: increased rate
- sporulation efficiency: decreased
- sporulation: absent
- starvation resistance: decreased
- stress resistance: increased
- transposable element transposition: increased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- repressible
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
474 total interactions for 375 unique genes
Physical Interactions
- Affinity Capture-MS: 49
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 20
- Biochemical Activity: 3
- Co-crystal Structure: 5
- Co-fractionation: 5
- Co-localization: 6
- PCA: 15
- Proximity Label-MS: 2
- Reconstituted Complex: 20
- Two-hybrid: 13
Genetic Interactions
- Negative Genetic: 245
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 3
- Positive Genetic: 55
- Synthetic Growth Defect: 22
- Synthetic Lethality: 3
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.