MIG3 / YER028C Overview
- Standard Name
- MIG3 1
- Systematic Name
- YER028C
- SGD ID
- SGD:S000000830
- Feature Type
- ORF , Verified
- Description
- Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes 1 2
- Name Description
- Multicopy Inhibitor of Growth 1
- Paralog
- MIG2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 394
- Mol. Weight (Da)
- 43140.0
- Isoelectric Point
- 10.55
- Median Abundance (molecules/cell)
- 1618 +/- 403
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MIG3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding transcription regulator involved in response to various stressors including UV and ethanol
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to UV (IGI)
- involved in DNA damage response (IGI, IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP, IGI)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in response to ethanol (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- chronological lifespan: decreased
- competitive fitness: decreased
- heat sensitivity: increased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- starvation resistance: decreased
- transposable element transposition: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The mig3 null mutant is viable; the null mutant of paralog mig2 is viable; the mig3 mig2 double mutant has not been annotated for phenotype.
41 total interactions for 37 unique genes
Physical Interactions
- Affinity Capture-MS: 2
- Affinity Capture-RNA: 3
- Biochemical Activity: 1
- Co-purification: 1
- Protein-RNA: 1
- Two-hybrid: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 3
- Negative Genetic: 21
- Positive Genetic: 3
- Synthetic Lethality: 1
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MIG3 encodes a Cys2His2 zinc-finger transcription factor of the beta-beta-alpha family. Mig3p binds 5'-CCCCRSV-3' motifs. Like Mig1p and Mig2p, Mig3p represses transcription of genes encoding enzymes for utilization of the sugars maltose, sucrose, or galactose when glucose is present. In some strains, this contribution to glucose repression by Mig3p is marginal. In other strains, Mig3p affects the expression of hundreds of glucose-responsive genes. Mig3p is involved in the transcriptional response to DNA damage, and represses the transcription of the SIR2 histone deacetylase involved in gene silencing and the control of aging. Mig3 is under dual level control by glucose. MIG3 transcription is induced by glucose, and Mig3p is subject to Snf1-dependent phosphorylation and subsequent degradation in the absence of glucose. MIG3 transcription is also induced by genotoxic stress, and Mig3p may function as a downstream effector in the Snf1-dependent response to hydroxyurea.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.