DNA2 / YHR164C Overview
- Standard Name
- DNA2 1
- Systematic Name
- YHR164C
- SGD ID
- SGD:S000001207
- Aliases
- WEB2
- Feature Type
- ORF , Verified
- Description
- ATP-dependent 5'-flap endonuclease and DNA helicase; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair/processing of meiotic DNA double strand breaks; cell cycle-dependent localization to telomeric chromatin and replicating chromosomes; required for telomerase-dependent telomere synthesis; stability, localization and nuclease activity regulated by sumoylation; forms nuclear foci upon replication stress; human homolog DNA2 complements yeast dna2 mutant 2 3 4 5 6 7 8 9 10 11 12 13 14 15
- Name Description
- DNA synthesis defective 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- DNA2/YHR164C is located on the right arm of chromosome VIII between SOL3 6-phosphogluconolactonase and PRP8 U4/U6-U5 snRNP complex subunit; coding sequence is 4569 nucleotides long with 47 SNPs, 23 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Dna2p is 1522 amino acids long, short-lived, extremely low in abundance; contains 2 helicase domains; sumoylated on 6 lysines, phosphorylated on 29 residues
- Length (a.a.)
- 1522
- Mol. Weight (Da)
- 171684.4
- Isoelectric Point
- 6.43
- Median Abundance (molecules/cell)
- 911 +/- 732
- Half-life (hr)
- 4.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DNA2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- 5'-flap endonuclease and ATP-dependent DNA helicase involved in lagging strand (Okazaki fragment) DNA elongation, DNA double-strand break processing, and telomere maintenance
View computational annotations
Molecular Function
- Manually Curated
- enables 5'-flap endonuclease activity (IDA)
- enables DNA hairpin binding (IDA, IMP)
- enables DNA helicase activity (IDA)
- enables four-way junction DNA binding (IDA)
- enables protein serine/threonine kinase activator activity (IDA, IMP)
- enables single-stranded DNA endodeoxyribonuclease activity (IDA)
- enables telomeric G-quadruplex DNA binding (IDA)
Biological Process
- Manually Curated
- involved in DNA double-strand break processing (IMP, IGI)
- involved in DNA repair (IMP)
- involved in DNA-templated DNA replication (IMP, IDA)
- involved in G-quadruplex DNA unwinding (IDA)
- involved in lagging strand elongation (IMP)
- involved in meiotic DNA double-strand break processing (IGI)
- involved in mitotic DNA replication checkpoint signaling (IGI)
- involved in telomere maintenance (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; repression causes chromosome instability; conditional mutations confer heat sensitivity, sensitivity to MMS and cadmium ions, and slow growth with shortened lifespan even at the permissive temperature
Classical Genetics
- repressible
- overexpression
- conditional
- null
- reduction of function
- unspecified
- null
- reduction of function
- overexpression
- repressible
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast DNA2 is homologous to human BLM, JAK2, WRN, and DNA2, and has been used to study mutations found in patients with Bloom syndrome, Werner syndrome, cancer, Seckel syndrome 8, and microcephalic primordial dwarfism
Manually Curated
- Bloom syndrome (ISS, IGI)
- cancer (ISS, IMP)
- microcephaly (ISS)
- Seckel syndrome (ISS, IMP)
- Seckel syndrome 8 (ISS, IMP)
- Werner syndrome (ISS, IGI)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Dna2p interacts physically with proteins involved in telomere organization; DNA2 interacts genetically with genes involved in DNA repair
855 total interactions for 577 unique genes
Physical Interactions
- Affinity Capture-MS: 21
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 9
- Biochemical Activity: 4
- Co-crystal Structure: 1
- Co-localization: 2
- Protein-peptide: 1
- Reconstituted Complex: 6
- Two-hybrid: 10
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 24
- Negative Genetic: 499
- Phenotypic Enhancement: 14
- Phenotypic Suppression: 32
- Positive Genetic: 109
- Synthetic Growth Defect: 37
- Synthetic Lethality: 50
- Synthetic Rescue: 33
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- DNA2 promoter is bound by Fkh1p during maintenance of stationary phase in response to starvation; DNA2 transcription is regulated by Sfp1p during stress response; Dna2 protein activity is upregulated by Nfi1p
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-09
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.