SCH9 / YHR205W Overview
- Standard Name
- SCH9 1
- Systematic Name
- YHR205W
- SGD ID
- SGD:S000001248
- Aliases
- KOM1 4 , HRM2 3
- Feature Type
- ORF , Verified
- Description
- AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan 2 3 4 5 6 7 8 9
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Sch9p is 824 amino acids long, somewhat short-lived, low in abundance; methylated on R182, ubiquitinylated on 3 lysines, phosphorylated on 38 residues
- Length (a.a.)
- 824
- Mol. Weight (Da)
- 91788.5
- Isoelectric Point
- 6.03
- Median Abundance (molecules/cell)
- 2752 +/- 1098
- Half-life (hr)
- 5.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SCH9 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine kinase involved in regulation of transcription by RNA polymerases I, II, and III; involved in regulation of sphingolipid biosynthesis, cell aging and responses to both oxidative and osmotic stresses; localizes to the nucleus, cytoplasm and vacuole membranes
View computational annotations
Molecular Function
- Manually Curated
- enables protein serine/threonine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in cellular response to oxidative stress (IMP)
- involved in positive regulation of hydrogen sulfide biosynthetic process (IMP)
- involved in positive regulation of ribosomal protein gene transcription by RNA polymerase II (IMP)
- involved in positive regulation of transcription by RNA polymerase I (IMP, IGI)
- involved in positive regulation of transcription by RNA polymerase III (IMP, IGI)
- involved in regulation of cell size (HMP)
- involved in regulation of cysteine metabolic process (IMP)
- involved in regulation of protein localization (IMP)
- involved in regulation of response to osmotic stress (IMP)
- involved in regulation of sphingolipid biosynthetic process (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants shows slow exponential growth, increased entry and survival in stationary phase, higher innate thermotolerance and longer replicative lifespan; null mutation decreases respiratory growth and increases resistance to cisplatin, myriocin and hydroxyurea, but lowers resistance to MMS and quinine; mutations affect sensitivity to hyperosmotic and oxidative stresses
Classical Genetics
- null
- acid pH resistance: increased
- apoptosis: decreased
- cell cycle progression in G1 phase: arrested
- cell size: decreased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: increased
- cold sensitivity: increased
- entry into G0 (stationary phase): increased
- hydrostatic pressure resistance: decreased
- hyperosmotic stress resistance: decreased
- hyperosmotic stress resistance: increased
- innate thermotolerance: increased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- mutation frequency: decreased
- osmotic stress resistance: decreased
- oxidative stress resistance: increased
- protein activity: increased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- protein/peptide modification: increased
- replicative lifespan: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased
- RNA accumulation: increased
- size of nucleus: decreased
- sporulation: decreased
- survival rate in stationary phase: decreased
- survival rate in stationary phase: increased
- UV resistance: decreased
- vegetative growth: absent
- vegetative growth: decreased
- repressible
- overexpression
- conditional
- unspecified
- activation
- null
- biofilm formation: decreased
- cell cycle progression in G1 phase: delayed
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: increased
- competitive fitness: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- ionic stress resistance: decreased
- metal resistance: decreased
- oxidative stress resistance: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- vegetative growth: decreased rate
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Sch9p interacts physically with proteins involved in transcription; SCH9 interacts genetically with genes involved in transcription
924 total interactions for 715 unique genes
Physical Interactions
- Affinity Capture-MS: 5
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 11
- Biochemical Activity: 32
- Co-localization: 1
- Co-purification: 1
- PCA: 4
- Protein-peptide: 1
- Proximity Label-MS: 2
- Reconstituted Complex: 21
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Lethality: 1
- Dosage Rescue: 12
- Negative Genetic: 286
- Phenotypic Enhancement: 26
- Phenotypic Suppression: 51
- Positive Genetic: 60
- Synthetic Growth Defect: 346
- Synthetic Lethality: 16
- Synthetic Rescue: 39
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SCH9 promoter is bound by Ste12p; SCH9 transcription is regulated by Gcn4p; Sch9p activity is regulated by Sgv1p during TORC1 signaling and by Snf1p during response to glucose starvation; Sch9p activity is downregulated by Npr1p during response to proline; Sch9p activity is upregulated by Npr2p during response to amino acid starvation and by TORC1 during stress response
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.