FLO11 / YIR019C Overview
- Standard Name
- FLO11 1
- Systematic Name
- YIR019C
- SGD ID
- SGD:S000001458
- Aliases
- STA4 , MUC1 3
- Feature Type
- ORF , Verified
- Description
- GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal and invasive growth, flocculation, and biofilm formation; major determinant of colony morphology; QTL that controls chronological life span; carries intragenic tandem repeats that are expanded in different strains; required for formation of fibrous interconnections between cells; role in co-flocculation with other yeast species; cleaved and shed from cells, contributing to their surface properties 2 3 4 5 6 7 8 9 10
- Name Description
- FLOcculation
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- Carries intragenic tandem repeats that are expanded in different strains
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1367
- Mol. Weight (Da)
- 136060.5
- Isoelectric Point
- 3.98
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FLO11 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein involved in flocculation, coflocculation, cell adhesion during biofilm formation, and in pseudohyphal and invasive growth; localizes to the plasma membrane, the bud neck and the extracellular region
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in cell adhesion involved in single-species biofilm formation (IMP)
- involved in cell-cell adhesion (IMP)
- involved in filamentous growth (IDA)
- involved in flocculation (IMP)
- involved in invasive growth in response to glucose limitation (IMP)
- involved in pseudohyphal growth (IMP)
- involved in single-species surface biofilm formation (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA)
- located in extracellular region (IDA)
- located in fungal-type vacuole (HDA)
- located in plasma membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation blocks biofilm formation, flocculation, and invasive growth, while overexpression increases those phenotypes; in large-scale studies, null mutant shows reduced competitive fitness in minimal and glycerol media
Classical Genetics
- overexpression
- unspecified
- null
- adhesion: absent
- adhesion: decreased
- biofilm formation: absent
- biofilm formation: decreased
- cell wall morphology: abnormal
- colony appearance: abnormal
- colony shape: abnormal
- filamentous growth: decreased
- flocculation: absent
- flocculation: decreased
- invasive growth: absent
- invasive growth: decreased
- pseudohyphal growth: absent
- resistance to chemicals: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vegetative growth: decreased
- reduction of function
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
137 total interactions for 117 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Affinity Capture-RNA: 3
- Proximity Label-MS: 1
- Reconstituted Complex: 2
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 61
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 51
- Positive Genetic: 10
- Synthetic Lethality: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 20
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-12
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.