HSL1 / YKL101W Overview
- Standard Name
- HSL1 1
- Systematic Name
- YKL101W
- SGD ID
- SGD:S000001584
- Aliases
- ELM2 9 , NIK1 10
- Feature Type
- ORF , Verified
- Description
- Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; phosphorylates and inhibits Pah1p, a phosphatidate phosphatase to regulate lipid synthesis; autophosphorylation enhances interactions with Hsl7p 2 3 4 5 6 7 8
- Name Description
- Histone Synthetic Lethal 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1518
- Mol. Weight (Da)
- 169646.3
- Isoelectric Point
- 9.93
- Median Abundance (molecules/cell)
- 2116 +/- 946
- Half-life (min)
- 58.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HSL1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ser/thr protein kinase involved in the G2/M transition of the mitotic cell cycle; substrates include Swe1p and Hsl7p; capable of autophosphorylation; localizes to the bud neck septin ring
View computational annotations
Molecular Function
- Manually Curated
- enables protein kinase activity (IDA, HDA)
- enables protein serine/threonine kinase activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in G2/M transition of mitotic cell cycle (IMP)
- involved in mitotic morphogenesis checkpoint signaling (IMP)
- involved in negative regulation of diacylglycerol biosynthetic process (IDA)
- involved in protein catabolic process (IMP)
- involved in protein localization to septin ring (IMP)
- involved in regulation of cell cycle (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA)
- located in cellular bud neck septin ring (IDA, IPI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- unspecified
- null
- overexpression
- bud morphology: abnormal
- bud neck morphology: abnormal
- budding index: decreased
- budding: abnormal
- cell cycle progression in G2 phase: abnormal
- cell cycle progression: abnormal
- cell size: increased
- cellular morphology: abnormal
- nuclear morphology: abnormal
- protein/peptide accumulation: decreased
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- null
- biofilm formation: increased
- cell cycle progression in G1 phase: decreased duration
- cell size: increased
- competitive fitness: decreased
- competitive fitness: increased
- metal resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation efficiency: increased
- toxin resistance: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
961 total interactions for 500 unique genes
Physical Interactions
- Affinity Capture-MS: 23
- Affinity Capture-RNA: 13
- Affinity Capture-Western: 18
- Biochemical Activity: 77
- Co-localization: 2
- PCA: 9
- Protein-peptide: 1
- Reconstituted Complex: 18
- Two-hybrid: 13
Genetic Interactions
- Dosage Growth Defect: 27
- Dosage Lethality: 1
- Dosage Rescue: 1
- Negative Genetic: 608
- Phenotypic Enhancement: 8
- Phenotypic Suppression: 8
- Positive Genetic: 86
- Synthetic Growth Defect: 11
- Synthetic Lethality: 21
- Synthetic Rescue: 16
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.