MLP1 / YKR095W Overview
- Standard Name
- MLP1 1
- Systematic Name
- YKR095W
- SGD ID
- SGD:S000001803
- Aliases
- MPL1
- Feature Type
- ORF , Verified
- Description
- Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex (NPC) with the nuclear interior; involved with Tel1p in telomere length control; forms a coiled-coil parallel homodimer; recruitment and docking of Mlp1p filaments onto the NPC Y-complex involves Nup60p; involved along with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication 1 2 3 4 5 6 7 8
- Name Description
- Myosin-Like Protein 1
- Paralog
- MLP2 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- MLP1 has a paralog, MLP2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1875
- Mol. Weight (Da)
- 218385.3
- Isoelectric Point
- 4.85
- Median Abundance (molecules/cell)
- 3413 +/- 1354
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MLP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ribonucleoprotein complex binding protein involved in protein localization to nuclear pore, telomere tethering at nuclear periphery, protein transport from cytoplasm to nucleus and negative regulation of protein import into nucleus during mitotic cell cycle spindle assembly checkpoint; localizes to nuclear envelope, nucleoplasm and spindle pole body
View computational annotations
Molecular Function
- Manually Curated
- enables mRNA binding (HDA)
- enables promoter-terminator loop anchoring activity (IMP)
- enables ribonucleoprotein complex binding (IGI, IPI)
Biological Process
- Manually Curated
- involved in negative regulation of protein import into nucleus during spindle assembly checkpoint (IGI)
- involved in nuclear mRNA surveillance (IMP, IGI)
- involved in poly(A)+ mRNA export from nucleus (IMP)
- involved in protein import into nucleus (IGI)
- involved in protein localization to nuclear pore (IMP)
- involved in telomere tethering at nuclear periphery (IGI)
Cellular Component
- Manually Curated
- located in nuclear envelope (IDA)
- part of nuclear pore nuclear basket (IDA)
- located in nucleoplasm (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- MLP1/YKR095W is a non-essential gene; null mutants are viable but exhibit abnormal nuclear export, abnormal protein/peptide distribution, abnormal vacuolar morphology, increased telomere length, increased toxin resistance, decreased UV resistance, and abnormal chemical compound accumulation. Overexpression of MLP1 results in increased RNA accumulation.
Classical Genetics
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast MLP1 is homologous to human TPR, and has been used to study lung cancer
Manually Curated
- lung cancer (ISS, IMP)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The mlp1 null mutant is viable; the null mutant of paralog mlp2 is viable; the mlp1 mlp2 double mutant is viable but exhibits clonal lethality.
528 total interactions for 334 unique genes
Physical Interactions
- Affinity Capture-MS: 180
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 14
- Biochemical Activity: 1
- Co-crystal Structure: 1
- Co-localization: 2
- PCA: 3
- Proximity Label-MS: 2
- Reconstituted Complex: 8
- Two-hybrid: 6
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 1
- Negative Genetic: 213
- Phenotypic Enhancement: 10
- Phenotypic Suppression: 5
- Positive Genetic: 38
- Synthetic Growth Defect: 15
- Synthetic Haploinsufficiency: 2
- Synthetic Lethality: 8
- Synthetic Rescue: 9
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.