ECO1 / YFR027W Overview
- Standard Name
- ECO1 1
- Systematic Name
- YFR027W
- SGD ID
- SGD:S000001923
- Aliases
- CTF7 16
- Feature Type
- ORF , Verified
- Description
- Acetyltransferase required for sister chromatid cohesion; elicits cohesin dimerization during S phase; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by Cdc28p, Cdc7p, Mck1p to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; relative distribution to nucleus increases upon DNA replication stress; mutations in human homolog ESCO2 cause Roberts syndrome 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
- Name Description
- Establishment of COhesion 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relative distribution to nucleus increases upon DNA replication stress
- Length (a.a.)
- 281
- Mol. Weight (Da)
- 31867.1
- Isoelectric Point
- 10.5
- Median Abundance (molecules/cell)
- 210 +/- 144
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ECO1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Acetyltransferase involved in chromatin looping, chromososme organization, DNA repair, DNA replication, telomere organization and tRNA gene clustering; localizes to chromatin, cytoplasm and nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables acetyltransferase activity (IDA, IMP)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in chromatin looping (IMP)
- involved in chromosome organization (IMP)
- involved in DNA repair (IDA)
- involved in DNA replication (IMP)
- involved in double-strand break repair (IMP)
- acts upstream of or within establishment of meiotic sister chromatid cohesion (IMP)
- involved in establishment of mitotic sister chromatid cohesion (IGI, IMP)
- involved in internal peptidyl-lysine acetylation (IDA)
- involved in mitotic chromosome condensation (IMP)
- involved in telomere organization (IMP)
- involved in tRNA gene clustering (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- ECO1/YFR027W is an essential gene; null mutants are inviable. Conditional mutants exhibit a range of phenotypic changes including arrested cell cycle progression through the G2/M phase transition, abnormal chromosome segregation and maintenance, decreased gamma ray and X-ray resistance, increased heat sensitivity, decreased mitotic recombination, abnormal nucleolar and spindle morphology, decreased oxidative stress resistance, decreased resistance to chemicals, altered RNA accumulation, decreased spore germination, decreased vegetative growth, and altered viability. Reduction of function mutants exhibit increased competitive fitness. Additionally, conditional mutants display increased colony sectoring and altered resistance to chemicals.
Classical Genetics
- conditional
- cell cycle progression through the G2/M phase transition: arrested
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chromosome segregation: abnormal
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- gamma ray resistance: decreased
- heat sensitivity: increased
- mitotic recombination: decreased
- nucleolar morphology: abnormal
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- RNA accumulation: decreased
- RNA accumulation: increased
- spindle morphology: abnormal
- spore germination: decreased
- vegetative growth: decreased
- viability: decreased
- viability: increased
- X ray resistance: decreased
- repressible
- unspecified
- reduction of function
- repressible
- conditional
- null
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast ECO1 is homologous to human ESCO2 and has been used to study Roberts syndrome, which is characterized by craniofacial anomalies, growth retardation, intellectual disability, and cardiac and renal abnormalities
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
300 total interactions for 157 unique genes
Physical Interactions
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 15
- Biochemical Activity: 24
- Protein-peptide: 1
- Reconstituted Complex: 6
- Two-hybrid: 6
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 15
- Negative Genetic: 123
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 16
- Positive Genetic: 8
- Synthetic Growth Defect: 15
- Synthetic Lethality: 30
- Synthetic Rescue: 33
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.