SIT4 / YDL047W Overview
- Standard Name
- SIT4 1
- Systematic Name
- YDL047W
- SGD ID
- SGD:S000002205
- Aliases
- PPH1 10
- Feature Type
- ORF , Verified
- Description
- Ceramide-activated, type 2A-related serine-threonine phosphatase; functions in G1/S transition of mitotic cycle; controls lifespan, mitochondrial function, cell cycle progression by regulating HXK2 phosphorylation; regulator of COPII coat dephosphorylation; required for ER to Golgi traffic; interacts with Hrr25p kinase; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization; similar to human PP6 2 3 4 5 6 7 8 9
- Name Description
- Suppressor of Initiation of Transcription 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 311
- Mol. Weight (Da)
- 35525.8
- Isoelectric Point
- 5.08
- Median Abundance (molecules/cell)
- 4289 +/- 1727
- Half-life (hr)
- 10.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SIT4 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine phosphatase involved in signal transduction, TOR signaling, and G1/S transition of the mitotic cell cycle; involved in DNA repair, replicative cell aging, tRNA wobble uridine modification, and organization of the actin cytoskeleton and cell wall; subunit of the protein phosphatase type 2A complex; localizes to the cytoplasm
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to oxidative stress (IMP)
- involved in DNA repair (IMP)
- involved in G1/S transition of mitotic cell cycle (IMP)
- involved in intracellular signal transduction (IMP)
- involved in regulation of actin cytoskeleton organization (IMP)
- involved in regulation of fungal-type cell wall organization (IMP)
- acts upstream of or within regulation of TORC1 signaling (IMP)
- involved in tRNA wobble uridine modification (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has fermentative and respiratory growth defects, increased heat sensitivity, abnormally elongated buds, extended chronological lifespan, decreased telomere length, and increased multidrug and toxin sensitivity; null mutants have semi-smooth colony morphology, altered mitochondrial morphology, a vacuolar fragmentation defect, decreased lipid droplet size, content and quantity and are sensitive to hyperosmotic stress; conditional mutant has an actin depolarization defect, is resistant to oxidants, and contains elevated levels of glutathione
Classical Genetics
- null
- apoptosis: increased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: increased
- cold sensitivity: increased
- fermentative metabolism: abnormal
- fermentative metabolism: decreased rate
- heat sensitivity: increased
- lipid particle morphology: abnormal
- metal resistance: decreased
- metal resistance: increased
- oxidative stress resistance: increased
- protein activity: increased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: abnormal
- protein/peptide modification: decreased
- protein/peptide modification: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- respiratory metabolism: abnormal
- respiratory metabolism: increased
- respiratory metabolism: increased rate
- RNA modification: abnormal
- RNA modification: decreased
- toxin resistance: increased
- utilization of carbon source: absent
- utilization of carbon source: decreased
- vegetative growth: decreased
- conditional
- overexpression
- null
- acid pH resistance: decreased
- biofilm formation: increased
- bud morphology: abnormal
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- colony appearance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- endoplasmic reticulum morphology: abnormal
- fermentative growth: decreased rate
- haploproficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- killer toxin resistance: decreased
- lipid particle morphology: abnormal
- metal resistance: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- stress resistance: decreased
- telomere length: decreased
- toxin resistance: decreased
- toxin resistance: increased
- transposable element transposition: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- repressible
- reduction of function
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
No Disease Ontology data available.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
349 total interactions for 206 unique genes
Physical Interactions
- Affinity Capture-MS: 114
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 30
- Biochemical Activity: 4
- Co-fractionation: 2
- Co-purification: 3
- PCA: 2
- Reconstituted Complex: 8
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 41
- Negative Genetic: 13
- Phenotypic Enhancement: 14
- Phenotypic Suppression: 28
- Positive Genetic: 19
- Synthetic Growth Defect: 11
- Synthetic Lethality: 30
- Synthetic Rescue: 25
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.