POL3 / YDL102W Overview
- Standard Name
- POL3 1
- Systematic Name
- YDL102W
- SGD ID
- SGD:S000002260
- Aliases
- CDC2 4 , HPR6 5 , TEX1 6
- Feature Type
- ORF , Verified
- Description
- Catalytic subunit of DNA polymerase delta; responsible for most of lagging strand DNA synthesis; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) 2 3
- Name Description
- POLymerase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- POL3/YDL102W is located in the left arm of chromosome IV between QRI1 UDP-N-acetylglucosamine pyrophosphorylase and DUN1 cell-cycle checkpoint serine/threonine protein kinase; coding sequence is 3294 nucleotides long with 20 SNPs, 4 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pol3p is 1097 amino acids long, short-lived, low in abundance; contains 3 DNA-directed DNA polymerase family B motifs; sumoylated on K13, ubiquitinylated on K1018, phosphorylated on 12 residues
- Length (a.a.)
- 1097
- Mol. Weight (Da)
- 124626.2
- Isoelectric Point
- 8.09
- Median Abundance (molecules/cell)
- 3354 +/- 1209
- Half-life (hr)
- 5.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all POL3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the delta DNA polymerase complex; has DNA polymerase and 3'-5'-exodeoxyribonuclease activities; involved in DNA replication, Okazaki initiator RNA removal, RNA-dependent DNA biosynthesis, and maintenance of fidelity during DNA replication
View computational annotations
Molecular Function
- Manually Curated
- enables 3'-5'-DNA exonuclease activity (IMP)
- enables DNA-directed DNA polymerase activity (IMP, IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in DNA replication (IMP)
- involved in DNA replication proofreading (IMP)
- involved in DNA replication, removal of RNA primer (IDA)
- involved in DNA-templated DNA replication maintenance of fidelity (IGI)
- involved in double-strand break repair via nonhomologous end joining (IGI, IMP)
- involved in RNA-templated DNA biosynthetic process (IDA)
Cellular Component
- Manually Curated
- part of delta DNA polymerase complex (TAS)
- located in replication fork (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; reduced function alleles confer increased mutation frequency, chromosome instability; conditional alleles arrest cell cycle progression, confer sensitivity to hydroxyurea and to MMS, resistance to camptothecin; in the heterozygous diploid with dominant negative alleles sporulation is normal but all spores are inviable; in the homozygous diploid conditional alleles arrest meiosis and interfere with sporulation
Classical Genetics
- conditional
- cell cycle progression in S phase: arrested
- cell cycle progression through the G2/M phase transition: arrested
- cell cycle progression: arrested
- cellular morphology: abnormal
- gamma ray resistance: decreased
- heat sensitivity: increased
- meiosis: arrested
- mutation frequency: increased
- sporulation: absent
- sporulation: decreased
- reduction of function
- unspecified
- dominant negative
- null
- null
- reduction of function
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast POL3 is homologous to human POLD1, and has been used to study mutations found in patients with colorectal and endometrial cancers
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pol3p interacts physically with proteins involved in DNA repair; POL3 interacts genetically with genes involved in DNA repair
1140 total interactions for 692 unique genes
Physical Interactions
- Affinity Capture-MS: 29
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 7
- Biochemical Activity: 1
- Co-crystal Structure: 1
- Co-localization: 1
- Co-purification: 5
- FRET: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 6
- Two-hybrid: 5
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 6
- Dosage Rescue: 11
- Negative Genetic: 716
- Phenotypic Enhancement: 46
- Phenotypic Suppression: 31
- Positive Genetic: 98
- Synthetic Growth Defect: 58
- Synthetic Lethality: 94
- Synthetic Rescue: 19
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- POL3 promoter is bound by Fkh1p in response to heat; POL3 transcription is regulated by Spt10p
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-03
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.