MSH6 / YDR097C Overview
- Standard Name
- MSH6
- Systematic Name
- YDR097C
- SGD ID
- SGD:S000002504
- Aliases
- PMS3 16 , PMS6 15
- Feature Type
- ORF , Verified
- Description
- Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p; contains PIP motif that binds PCNA (Pol30p) and Rev1p 1 2 3 4 5
- Name Description
- MutS Homolog
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein is 1242 amino acids long with 5 MutS domains; subunit of the DNA mismatch repair MutSalpha complex; shares many domains with DNA repair proteins Msh1p and Msh3p; phosphorylated at many residues in N-terminal portion of protein, also sumoylated and acetylated at residues in central portion of protein
- Length (a.a.)
- 1242
- Mol. Weight (Da)
- 140058.8
- Isoelectric Point
- 5.56
- Median Abundance (molecules/cell)
- 2793 +/- 1985
- Half-life (hr)
- 7.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MSH6 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATP binding involved in interstrand cross-link repair, meiotic mismatch repair and replication fork arrest; subunit of MutSalpha localizes to cytoplasm and nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables ATP binding (IDA)
- enables ATP hydrolysis activity (IDA)
- enables four-way junction DNA binding (IDA)
- contributes to guanine/thymine mispair binding (IDA)
- contributes to single base insertion or deletion binding (IDA, IMP)
Biological Process
- Manually Curated
- involved in interstrand cross-link repair (IGI)
- involved in meiotic mismatch repair (IMP)
- involved in mismatch repair (IMP, IDA)
- involved in replication fork arrest (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene, null mutant shows defect in vacuolar fragmentation, increased mutation frequency, spontaneous direct-repeat hyperrecombination, decreased chronological life span, and a significant increase in actin cable abundance
Classical Genetics
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- mutation frequency: increased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast MSH6 is homologous to human MSH6, and has been used to study mutations found in patients with colorectal, breast, and ovarian cancers; inactivation of the MMR system causes genetic instability and strongly predisposes humans to the development of cancers
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Interacts physically with proteins involved in DNA repair and meiosis; interacts genetically with genes implicated in DNA repair and replication
271 total interactions for 133 unique genes
Physical Interactions
- Affinity Capture-MS: 65
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 4
- Biochemical Activity: 2
- Co-fractionation: 5
- Co-purification: 11
- FRET: 1
- Protein-peptide: 1
- Reconstituted Complex: 30
- Two-hybrid: 7
Genetic Interactions
- Dosage Growth Defect: 4
- Dosage Lethality: 1
- Dosage Rescue: 5
- Negative Genetic: 47
- Phenotypic Enhancement: 51
- Phenotypic Suppression: 8
- Positive Genetic: 8
- Synthetic Growth Defect: 3
- Synthetic Lethality: 8
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Transcription of MSH6 is regulated by Sfp1p in response to stress, and also by Mbp1p; protein activity is regulated by Cdc28p
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-09-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.