BMH2 / YDR099W Overview
- Standard Name
- BMH2 1
- Systematic Name
- YDR099W
- SGD ID
- SGD:S000002506
- Aliases
- SCD3 8
- Feature Type
- ORF , Verified
- Description
- 14-3-3 protein, minor isoform; involved in post-transcriptional control of the proteome; binds to both proteins and to DNA, including replication origins; regulates multiple processes including exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling and meiotic commitment; protein abundance and relative distribution to the nucleus increase upon DNA replication stress; abundance relative to Bmh1p increases during sporulation; similar to several human 14-3-3 proteins 2 3 5 6 7
- Name Description
- Brain Modulosignalin Homolog 4
- Paralog
- BMH1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- 14-3-3 protein, minor isoform; protein abundance and relative distribution to the nucleus increase upon DNA replication stress; abundance relative to Bmh1p increases during sporulation
- Length (a.a.)
- 273
- Mol. Weight (Da)
- 31045.5
- Isoelectric Point
- 4.54
- Median Abundance (molecules/cell)
- 42982 +/- 10894
- Half-life (hr)
- 10.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BMH2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Phosphoserine- and DNA replication origin-binding protein involved in Ras protein signal transduction and signal transduction during filamentous growth; involved in many processes including DNA replication initiation, DNA damage checkpoint, negative regulation of apoptosis, and cell wall chitin biosynthesis; localizes to the nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables DNA replication origin binding (IDA)
- enables phosphoserine residue binding (IMP)
Biological Process
- Manually Curated
- involved in ascospore formation (IGI)
- involved in chitin biosynthetic process (IGI)
- involved in DNA damage checkpoint signaling (IMP)
- involved in DNA replication initiation (IGI)
- involved in mitotic cell cycle (IPI)
- involved in negative regulation of apoptotic process (IMP)
- involved in negative regulation of protein ubiquitination (IPI)
- involved in pre-replicative complex assembly involved in nuclear cell cycle DNA replication (IGI)
- involved in pseudohyphal growth (IGI)
- involved in Ras protein signal transduction (IGI)
- involved in regulation of glycogen metabolic process (IGI)
- involved in signal transduction involved in filamentous growth (IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation results in increased frequency of gross chromosomal rearrangements and lengthened chronological lifespan; null mutant is sensitive to selenite and resistant to selenomethionine; overexpression leads to slow growth and actin cytoskeleton abnormalities
Classical Genetics
- null
- overexpression
- actin cytoskeleton morphology: abnormal
- cell shape: abnormal
- chronological lifespan: increased
- hyperosmotic stress resistance: decreased
- invasive growth: increased
- protein/peptide accumulation: abnormal
- resistance to chemicals: increased
- RNA accumulation: increased
- vegetative growth: decreased
- vegetative growth: decreased rate
- null
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The bmh2 null mutant is viable; the null mutant of paralog bmh1 is viable; the bmh2 bmh1 double mutant is inviable or displays a growth defect.
471 total interactions for 330 unique genes
Physical Interactions
- Affinity Capture-MS: 196
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 41
- Biochemical Activity: 7
- Co-fractionation: 1
- Co-purification: 3
- Far Western: 1
- PCA: 3
- Protein-peptide: 5
- Proximity Label-MS: 5
- Reconstituted Complex: 11
- Two-hybrid: 29
Genetic Interactions
- Dosage Growth Defect: 14
- Dosage Lethality: 3
- Dosage Rescue: 9
- Negative Genetic: 94
- Phenotypic Enhancement: 10
- Phenotypic Suppression: 3
- Positive Genetic: 8
- Synthetic Growth Defect: 10
- Synthetic Lethality: 8
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-09-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.