RAD9 / YDR217C Overview
- Standard Name
- RAD9 1
- Systematic Name
- YDR217C
- SGD ID
- SGD:S000002625
- Feature Type
- ORF , Verified
- Description
- DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; protects double-strand breaks from premature resection; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p 2 3 4 5 6 7
- Name Description
- RADiation sensitive 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1309
- Mol. Weight (Da)
- 148357.0
- Isoelectric Point
- 4.96
- Median Abundance (molecules/cell)
- 1279 +/- 701
- Half-life (hr)
- 1.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RAD9 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Double-stranded DNA binding protein; involved in response to DNA damage; plays a role in the DNA damage checkpoint at G1 and intra-S phases; localizes in the chromatin
View computational annotations
Molecular Function
- Manually Curated
- enables double-stranded DNA binding (IDA)
- enables enzyme activator activity (IMP)
- enables histone binding (IDA)
Biological Process
- Manually Curated
- involved in DNA damage checkpoint signaling (IMP)
- involved in DNA repair (IGI, IMP)
- involved in double-strand break repair (IMP)
- involved in mitotic G1 DNA damage checkpoint signaling (IMP)
- involved in mitotic intra-S DNA damage checkpoint signaling (IMP)
- involved in negative regulation of DNA strand resection involved in replication fork processing (IMP)
- involved in nucleotide-excision repair (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in regulation of cell cycle (IMP, IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sensitive to DNA damaging agents, DNA synthesis and topoisomerase inhibitors; null mutant is sensitive to X-rays, gamma rays and UV radiation; null mutant displays increased chromosome instability including gross chromosomal rearrangements, but decreased reversion rate in the presence of high doses of DNA damaging agents; null mutant displays elevated levels of sumoylated Rfa1p, Rfa2p, and Rad52p after DNA damage; null mutant has a decreased duration of S phase and impaired growth on non-fermentative carbon sources, decreased oxidative stress resistance, and decreased chronological lifespan; overexpression results in a decreased rate of vegetative growth
Classical Genetics
- null
- cell cycle progression in S phase: decreased duration
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- chronological lifespan: decreased
- gamma ray resistance: decreased
- metal resistance: decreased
- mutation frequency: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: increased
- radiation resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory metabolism: decreased
- RNA accumulation: decreased
- RNA accumulation: increased
- UV resistance: decreased
- viable
- X ray resistance: decreased
- reduction of function
- unspecified
- null
- chemical compound accumulation: abnormal
- chromosome/plasmid maintenance: abnormal
- competitive fitness: decreased
- gamma ray resistance: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: increased
- UV resistance: decreased
- viable
- X ray resistance: decreased
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rad9p interacts physically with proteins involved in regulation of cell cycle; RAD9 interacts genetically with genes involved in DNA repair
1060 total interactions for 502 unique genes
Physical Interactions
- Affinity Capture-MS: 9
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 28
- Biochemical Activity: 6
- Co-localization: 2
- Co-purification: 1
- PCA: 1
- Protein-peptide: 4
- Reconstituted Complex: 10
- Two-hybrid: 13
Genetic Interactions
- Dosage Growth Defect: 4
- Dosage Lethality: 2
- Dosage Rescue: 2
- Negative Genetic: 253
- Phenotypic Enhancement: 286
- Phenotypic Suppression: 107
- Positive Genetic: 44
- Synthetic Growth Defect: 129
- Synthetic Lethality: 57
- Synthetic Rescue: 97
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-03-30
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.