SPT3 / YDR392W Overview
- Standard Name
- SPT3 1
- Systematic Name
- YDR392W
- SGD ID
- SGD:S000002800
- Feature Type
- ORF , Verified
- Description
- Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; role in preventing L-A mycovirus pathogenesis; relocalizes to the cytosol in response to hypoxia 2 3 4 5 6
- Name Description
- SuPpressor of Ty's 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 337
- Mol. Weight (Da)
- 38801.6
- Isoelectric Point
- 8.25
- Median Abundance (molecules/cell)
- 1593 +/- 576
- Half-life (hr)
- 8.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SPT3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription cofactor subunit of SAGA and SLIK histone acetyltransferase complexes; involved in chromatin modification and regulates transcription by RNA polymerase II related to invasive growth in response to glucose limitation
View computational annotations
Molecular Function
- Manually Curated
- enables transcription coregulator activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in chromatin organization (IDA)
- involved in invasive growth in response to glucose limitation (IDA)
- involved in pseudohyphal growth (IDA)
- involved in transcription by RNA polymerase II (IMP)
Cellular Component
- Manually Curated
- located in cytosol (IDA)
- located in nucleus (IDA)
- part of SAGA complex (IDA)
- part of SLIK (SAGA-like) complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants grow slowly, have abnormal vacuolar morphology, decreased ethanol tolerance, decreased transposition, small cell size, are sensitive to heat and dessication, and auxotrophic for myo-inositol; homozygous diploid nulls are defective in sporulation
Classical Genetics
- null
- chemical compound accumulation: decreased
- chronological lifespan: decreased
- osmotic stress resistance: decreased
- protein/peptide accumulation: decreased
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- subcellular morphology: abnormal
- transposable element transposition: decreased
- utilization of carbon source: absent
- null
- acquired thermotolerance: decreased
- auxotrophy
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- heat sensitivity: increased
- innate thermotolerance: decreased
- killer toxin resistance: decreased
- metal resistance: decreased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: abnormal
- sporulation efficiency: decreased
- sporulation: decreased
- stress resistance: decreased
- toxin resistance: increased
- transposable element transposition: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
952 total interactions for 519 unique genes
Physical Interactions
- Affinity Capture-MS: 115
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 39
- Co-fractionation: 6
- Co-localization: 3
- Co-purification: 30
- PCA: 2
- Reconstituted Complex: 8
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 3
- Negative Genetic: 481
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 4
- Positive Genetic: 91
- Synthetic Growth Defect: 74
- Synthetic Lethality: 58
- Synthetic Rescue: 20
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SPT3 encodes a subunit of SAGA, a multi-subunit complex that either activates or inhibits transcription of some RNA polymerase II-dependent genes. In its role as an activator, Spt3p interacts with the TATA-binding protein (TBP), encoded by SPT15, and has been shown to be required for recruitment of TBP to the SAGA-dependent promoters GAL1, VTC3, BDF2, PHO84, and ADH1. Studies of activation at the GAL1 promoter indicate that the activator protein Gal4p recruits SAGA to the upstream activation sequence (UAS), and that SAGA subsequently recruits TBP via Spt3p and Spt20p. During SAGA-mediated transcriptional inhibition, which has been demonstrated at the HIS3 and TRP3 promoters, Spt3p and Spt8p prevent TBP binding to the TATA box which presumably inhibits transcription.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2003-04-04
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.