PDR1 / YGL013C Overview
- Standard Name
- PDR1 1
- Systematic Name
- YGL013C
- SGD ID
- SGD:S000002981
- Aliases
- AMY1 19 , ANT1 20 , BOR2 21 , CYH3 22 , NRA2 , SMR2 , TIL1 , TPE1 23 , TPE3
- Feature Type
- ORF , Verified
- Description
- Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication 2 3 4
- Name Description
- Pleiotropic Drug Resistance 1
- Paralog
- PDR3 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- PDR1 has a paralog, PDR3, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 1068
- Mol. Weight (Da)
- 121789.0
- Isoelectric Point
- 6.93
- Median Abundance (molecules/cell)
- 1597 +/- 617
- Half-life (hr)
- 11.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PDR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence specific DNA-binding polymerase II transcription factor that activates expression of genes involved in drug response
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to xenobiotic stimulus (IMP)
- involved in positive regulation of cellular response to drug (IMP, IGI)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null and point mutations confer resistance or sensitivity to various drugs; in large-scale studies, null mutation confers increased competitive fitness in glycerol medium, decreased fitness in minimal medium, and altered resistance to a wide variety of drugs; overexpression causes slow growth
Classical Genetics
- null
- gain of function
- unspecified
- overexpression
- null
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The pdr1 null mutant is viable; the null mutant of paralog pdr3 is viable; the pdr1 pdr3 double mutant displays a synthetic growth defect.
357 total interactions for 260 unique genes
Physical Interactions
- Affinity Capture-MS: 72
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 8
- Biochemical Activity: 1
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-localization: 1
- PCA: 3
- Proximity Label-MS: 1
- Reconstituted Complex: 3
- Two-hybrid: 7
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 3
- Negative Genetic: 165
- Phenotypic Enhancement: 33
- Phenotypic Suppression: 6
- Positive Genetic: 16
- Synthetic Growth Defect: 27
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- PDR1 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Pdr1p activates transcription of genes involved in pleiotropic drug resistance, such as the ATP-binding cassette transporter genes PDR5, SNQ2, and YOR1, whose products mediate drug efflux from the cell. Pdr1p targets also have roles in hydroxymethylfurfural (HMF) tolerance, ethanol tolerance, the salt stress response, and sphingolipid biosynthesis. Pdr1p binds to multiple PDREs (pleiotropic drug resistance elements) in the promoters of target genes, either as a homodimer or as a heterodimer with other transcription factors, including Pdr3p and Stb5p. PDR3, a paralog of PDR1, is also a target of Pdr1p regulation. Pdr1p is phosphorylated, although the regulatory significance of this is unknown.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-09-28
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.