MIG1 / YGL035C Overview
- Standard Name
- MIG1 1
- Systematic Name
- YGL035C
- SGD ID
- SGD:S000003003
- Aliases
- SSN1 10 , CAT4 11 , TDS22
- Feature Type
- ORF , Verified
- Description
- Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state 1 2 3 4 5 6 7
- Name Description
- Multicopy Inhibitor of GAL gene expression 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mig1p is 504 amino acid long, very short-lived, low in abundance; has 2 C2H2-type zinc fingers; phosphorylated on 44 residues
- Length (a.a.)
- 504
- Mol. Weight (Da)
- 55550.0
- Isoelectric Point
- 10.43
- Median Abundance (molecules/cell)
- 2001 +/- 648
- Half-life (hr)
- 3.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MIG1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to glucose and starvation
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription repressor activity, RNA polymerase II-specific (IGI, IDA, IMP)
- enables RNA polymerase II cis-regulatory region sequence-specific DNA binding (IDA)
- enables sequence-specific DNA binding (HDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null muants exhibit accelerated growth, decreased replicative lifespan, increased chronological lifespan, decreased ethanol tolerance, have abnormal vacuolar morphology, are tolerant of zinc deficiency and of heat shock; overexpression shortens replicative lifespan, slows vegetative growth, enhances filamentous growth, interferes with cell cycle progression and budding
Classical Genetics
- null
- acid pH resistance: increased
- alkaline pH resistance: decreased
- alkaline pH resistance: increased
- biofilm formation: decreased
- chemical compound accumulation: increased
- desiccation resistance: decreased
- replicative lifespan: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vegetative growth: increased
- viable
- overexpression
- overexpression
- null
- anaerobic growth: decreased
- chemical compound accumulation: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- innate thermotolerance: increased
- oxidative stress resistance: decreased
- protein activity: increased
- resistance to chemicals: decreased
- stress resistance: increased
- toxin resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: increased rate
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Mig1p interacts physically with proteins involved in transcription; MIG1 interacts genetically with genes involved in transcription
384 total interactions for 274 unique genes
Physical Interactions
- Affinity Capture-MS: 16
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 9
- Biochemical Activity: 17
- PCA: 1
- Reconstituted Complex: 6
- Two-hybrid: 19
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 3
- Dosage Rescue: 18
- Negative Genetic: 190
- Phenotypic Enhancement: 26
- Phenotypic Suppression: 16
- Positive Genetic: 21
- Synthetic Growth Defect: 5
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 1
- Synthetic Rescue: 25
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MIG1 encodes a Cys2His2 zinc-finger transcription factor of the beta-beta-alpha family. Mig1p is the main effector in glucose repression of SUC, MAL and GAL genes, and binds 5'-MCCCCRS-3' motifs. Like Mig2p, Mig1p represses transcription of the genes encoding enzymes for utilization of the sugars maltose, sucrose, or galactose when glucose is present. Repression can be both direct and indirect, through repression of genes encoding transcriptional activators. GAL genes are repressed directly by Mig1p, and indirectly through repression of the GAL4 gene. During repressing conditions, cytoplasmic Mig1p is dephosphorylated by the Reg1p-Glc7p protein phosphatase complex, then imported into the nucleus where it binds promoters of glucose-repressed genes and recruits the Cyc8p-Tup1p corepressor. Under glucose limitation, Mig1p is deactivated via phosphorylation by the Snf1 kinase complex at four sites (serines 222, 278, 311, 381), then exported from the nucleus by exportin Msn5p. The central amino acids 217-400 of Mig1p, containing the Snf1 phosphorylation sites, is the transport domain, with nuclear export and nuclear localization sequences.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-02-16
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.