GUP1 / YGL084C Overview
- Standard Name
- GUP1 1
- Systematic Name
- YGL084C
- SGD ID
- SGD:S000003052
- Feature Type
- ORF , Verified
- Description
- Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; role in misfolded protein quality control; proposed to be involved in glycerol transport; homolog of the mammalian Hedgehog pathway modulator HHATL; GUP1 has a paralog, GUP2, that arose from the whole genome duplication 2 3 4 5 6 7 8
- Name Description
- Glycerol UPtake 1
- Paralog
- GUP2 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- GUP1 has a paralog, GUP2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 560
- Mol. Weight (Da)
- 65359.4
- Isoelectric Point
- 9.82
- Median Abundance (molecules/cell)
- 2305 +/- 1226
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GUP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- O-acyltransferase involved in glycerol transport and catabolism, and GPI anchor biosynthesis; localizes to plasma membrane and endoplasmic reticulum
View computational annotations
Molecular Function
- Manually Curated
- enables O-acyltransferase activity (IMP)
Biological Process
- Manually Curated
- involved in glycerol catabolic process (IMP)
- involved in glycerol transmembrane transport (IMP)
- involved in GPI anchor biosynthetic process (IMP)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (IDA, HDA)
- located in plasma membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- GUP1/YGL084C is a non-essential gene; null mutants are viable but exhibit a range of phenotypes including decreased anaerobic growth, abnormal budding pattern, abnormal cell shape, decreased cell size, abnormal cell wall morphology, increased chitin deposition, decreased chronological lifespan, decreased competitive fitness, decreased endocytosis, increased heat sensitivity, decreased hyperosmotic stress resistance, abnormal liquid culture appearance, decreased metal resistance, increased necrotic cell death, decreased nutrient uptake, increased oxidative stress resistance, abnormal plasma membrane morphology, absent or decreased rate of respiratory growth, increased RNA accumulation, decreased small molecule transport, decreased telomere length, decreased toxin resistance, and abnormal vacuolar morphology. Overexpression of GUP1 results in abnormal endomembrane system morphology.
Classical Genetics
- null
- cell death: increased
- cell wall morphology: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chitin deposition: increased
- chronological lifespan: decreased
- heat sensitivity: increased
- heat sensitivity: normal
- hyperosmotic stress resistance: decreased
- liquid culture appearance: abnormal
- metal resistance: decreased
- necrotic cell death: increased
- nutrient uptake: decreased
- plasma membrane morphology: abnormal
- protein activity: decreased
- protein transport: abnormal
- protein/peptide accumulation: decreased
- protein/peptide distribution: decreased
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- RNA accumulation: increased
- utilization of carbon source: decreased
- vegetative growth: decreased
- overexpression
- null
- anaerobic growth: decreased
- budding pattern: abnormal
- cell shape: abnormal
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chitin deposition: increased
- chromosome/plasmid maintenance: decreased
- competitive fitness: decreased
- endocytosis: decreased
- haploinsufficient
- heat sensitivity: increased
- metal resistance: decreased
- oxidative stress resistance: increased
- protein transport: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- respiratory growth: decreased rate
- small molecule transport: decreased
- telomere length: decreased
- toxin resistance: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: absent
- vegetative growth: decreased rate
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The gup1 null mutant is viable in the S288C background and inviable in the W303 background; the null mutant of paralog gup2 is viable; the gup1 gup2 double mutant displays a phentoypic enhancement.
853 total interactions for 508 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 4
- Biochemical Activity: 2
- PCA: 4
- Proximity Label-MS: 1
- Two-hybrid: 2
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Rescue: 1
- Negative Genetic: 618
- Phenotypic Enhancement: 6
- Positive Genetic: 151
- Synthetic Growth Defect: 30
- Synthetic Lethality: 17
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.