BRR6 / YGL247W Overview
- Standard Name
- BRR6 1
- Systematic Name
- YGL247W
- SGD ID
- SGD:S000003216
- Feature Type
- ORF , Verified
- Description
- Essential nuclear envelope integral membrane protein; interacts with chromatin, helping to maintain normal chromatin architecture and transcriptional regulation of certain loci at the nuclear envelope; interacts and functions with Apq12p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA nuclear export, and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; homologous to Brl1p 1 2 4 5
- Name Description
- Bad Response to Refrigeration 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 197
- Mol. Weight (Da)
- 22809.6
- Isoelectric Point
- 8.32
- Median Abundance (molecules/cell)
- 2516 +/- 1003
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BRR6 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Integral membrane protein of the nuclear envelope and ER; involved in lipid homeostasis; role in nuclear export of mRNA, and nuclear envelope organization
View computational annotations
Molecular Function
- Manually Curated
- enables chromatin binding (IDA)
Biological Process
- Manually Curated
- involved in lipid homeostasis (IGI, IMP)
- involved in nuclear envelope organization (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
Cellular Component
- Manually Curated
- located in cell periphery (HDA)
- located in endoplasmic reticulum (HDA)
- is active in nuclear envelope (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutant is cold sensitive, some alleles are also heat sensitive, and slow growing at permissive temperature; conditional mutant is sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents, accumulating ergosterol, sterol precursors and monounsaturated fatty acids at reduced temperatures; conditional mutant is defective in the export of poly(A)+ mRNA, and mislocalizes nuclear pore complex (NPC) cytoplasmic filament proteins; conditional mutant has a nuclear morphology defect with abnormal nuclear envelope, and an abnormal lipid droplet arrangement with a circular patter of droplets surrounding the nucleus; overexpression results in a decreased growth rate
Classical Genetics
- conditional
- cell cycle progression in M phase: arrested
- chemical compound accumulation: increased
- cold sensitivity: increased
- cytoskeleton morphology: abnormal
- heat sensitivity: increased
- lipid particle morphology: abnormal
- nuclear export: decreased
- nuclear morphology: abnormal
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- spindle morphology: abnormal
- vegetative growth: decreased
- null
- overexpression
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
450 total interactions for 362 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 7
- Co-localization: 1
- PCA: 2
- Two-hybrid: 3
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 4
- Negative Genetic: 346
- Phenotypic Enhancement: 1
- Positive Genetic: 63
- Synthetic Lethality: 16
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.