HXK2 / YGL253W Overview
- Standard Name
- HXK2 1
- Systematic Name
- YGL253W
- SGD ID
- SGD:S000003222
- Aliases
- HEX1 21 , HKB 22 , SCI2
- Feature Type
- ORF , Verified
- Description
- Hexokinase isoenzyme 2; phosphorylates glucose in cytosol; predominant hexokinase during growth on glucose; represses expression of HXK1, GLK1, induces expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at Ser14 by kinase Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; functions downstream of Sit4p in control of cell cycle, mitochondrial function, oxidative stress resistance, chronological lifespan; has paralog HXK1 2 3 4 5 6 7
- Name Description
- HeXoKinase
- Paralog
- HXK1 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 486
- Mol. Weight (Da)
- 53930.9
- Isoelectric Point
- 4.93
- Median Abundance (molecules/cell)
- 136905 +/- 64001
- Half-life (hr)
- 17.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HXK2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Hexokinase, phosphorylates D-hexoses to D-hexose-6-phosphates; involved in cellular metabolism and homeostasis of glucose, fructose and mannose; localized to cytosol, nucleus and mitochondria
View computational annotations
Molecular Function
- Manually Curated
- enables hexokinase activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in D-glucose import (IGI)
- involved in fructose import across plasma membrane (IGI)
- involved in fructose metabolic process (IMP)
- involved in glucose metabolic process (IMP)
- involved in glycolytic process (IMP, IDA)
- involved in mannose metabolic process (IDA)
- involved in negative regulation of apoptotic signaling pathway (IMP)
- involved in regulation of cell size (HMP)
- involved in regulation of transcription by glucose (IDA)
Cellular Component
- Manually Curated
- located in cytosol (IDA)
- located in mitochondrial membrane (IMP)
- located in mitochondrion (IDA, HDA)
- located in nucleus (IDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays reduced growth rate, decreased replicative lifespan and increased apoptosis; null mutation also causes sensitivity to alkaline pH, acetic acid, fluconazole, but increased resistance to caffeine, camptothecin, bleomycin
Classical Genetics
- null
- alkaline pH resistance: decreased
- apoptosis: increased
- cell size: decreased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- desiccation resistance: decreased
- fermentative metabolism: decreased rate
- growth in exponential phase: decreased rate
- protein activity: decreased
- protein activity: increased
- protein/peptide modification: decreased
- replicative lifespan: decreased
- replicative lifespan: increased
- replicative lifespan: normal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: decreased
- RNA accumulation: increased
- utilization of carbon source: decreased rate
- vegetative growth: decreased rate
- reduction of function
- misexpression
- null
- bud morphology: abnormal
- cell size: decreased
- chromosome/plasmid maintenance: decreased
- colony sectoring: increased
- competitive fitness: decreased
- competitive fitness: increased
- fermentative growth: decreased rate
- innate thermotolerance: increased
- metal resistance: increased
- oxidative stress resistance: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: increased
- toxin resistance: increased
- vegetative growth: decreased rate
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The hxk2 null mutant is viable; the null mutant of paralog hxk1 is viable; the hxk2 hxk1 double mutant is inviable or displays a growth defect.
498 total interactions for 327 unique genes
Physical Interactions
- Affinity Capture-MS: 54
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 15
- Biochemical Activity: 2
- Co-purification: 2
- PCA: 3
- Proximity Label-MS: 2
- Reconstituted Complex: 10
- Two-hybrid: 6
Genetic Interactions
- Negative Genetic: 283
- Phenotypic Enhancement: 26
- Phenotypic Suppression: 10
- Positive Genetic: 51
- Synthetic Growth Defect: 10
- Synthetic Lethality: 5
- Synthetic Rescue: 11
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 16
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-11-29
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.