TIF4631 / YGR162W Overview
- Standard Name
- TIF4631 1 2
- Systematic Name
- YGR162W
- SGD ID
- SGD:S000003394
- Aliases
- eIF4G1 8
- Feature Type
- ORF , Verified
- Description
- Translation initiation factor eIF4G and scaffold protein; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E (Cdc33p) and eIF4A (Tif1p/Tif2p); stimulates the ATPase and helicase activities of eIF4A synergistically with eIF4G; required with eIF4E for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; association with Pab1p mediates poly(A) tail-stimulated translation; role in assembly of PET region in biogenesis of 60S ribosomal subunit 1 3 4 5 6 7 8 9 10 11
- Name Description
- Translation Initiation Factor 1 2
- Paralog
- TIF4632
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 952
- Mol. Weight (Da)
- 107088.7
- Isoelectric Point
- 5.84
- Median Abundance (molecules/cell)
- 21078 +/- 10600
- Half-life (hr)
- 9.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TIF4631 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Translation initiation factor and ATPase activator involved in translational initiation, stress granule assembly in response to starvation, and ribosomal large subunit biogenesis; stimulates the ATPase and helicase activities of eIF4A; stimulates formation of the 48S preinitiation complex (PIC); subunit of the eukaryotic translation initiation factor 4F complex; localizes to the cytoplasm, cytoplasmic stress granule, and cytoplasmic mRNA processing body
View computational annotations
Molecular Function
- Manually Curated
- enables ATPase activator activity (IDA)
- enables mRNA binding (HDA)
- enables protein binding (IPI)
- enables RNA binding (IDA)
Biological Process
- Manually Curated
- involved in cellular response to glucose starvation (IMP)
- involved in positive regulation of formation of translation preinitiation complex (IDA)
- involved in ribosomal large subunit biogenesis (IMP)
- involved in stress granule assembly (IMP)
- involved in translational initiation (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in cytoplasmic stress granule (IDA, HDA)
- part of eukaryotic translation initiation factor 4F complex (IMP)
- located in mitochondrion (HDA)
- located in P-body (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- TIF4631/YGR162W null mutants exhibit increased cold and heat sensitivity, decreased nuclear export, decreased protein/peptide accumulation, increased replicative lifespan, altered resistance to various chemicals, and decreased vegetative growth rate. They also show auxotrophy, abnormal bud morphology, delayed cell cycle progression in the G1 phase, abnormal and decreased desiccation resistance, slow growth, increased oxidative stress resistance, absent or decreased sporulation, increased stress resistance, decreased toxin resistance, and increased transposable element transposition. Conditional mutants of TIF4631 display increased cold and heat sensitivity and decreased vegetative growth. Overexpression of TIF4631 results in decreased growth rate during the exponential phase, decreased vegetative growth rate, abnormal cell cycle progression, decreased competitive fitness, increased heat sensitivity, decreased hyperosmotic stress resistance, increased invasive growth, decreased metal resistance, decreased resistance to chemicals, increased toxin resistance, and decreased UV resistance. Reduction of function mutants show increased heat sensitivity and decreased vegetative growth.
Classical Genetics
- conditional
- null
- overexpression
- reduction of function
- null
- auxotrophy
- bud morphology: abnormal
- cell cycle progression in G1 phase: delayed
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- fermentative growth: decreased rate
- haploinsufficient
- haploproficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- metal resistance: decreased
- oxidative stress resistance: increased
- protein/peptide accumulation: increased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: increased
- sporulation: absent
- sporulation: decreased
- stress resistance: increased
- toxin resistance: decreased
- transposable element transposition: increased
- vegetative growth: decreased rate
- overexpression
- cell cycle progression: abnormal
- competitive fitness: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- invasive growth: increased
- metal resistance: decreased
- resistance to chemicals: decreased
- toxin resistance: increased
- UV resistance: decreased
- vegetative growth: decreased rate
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast TIF4631 is homologous to human EIF4G1, and has been used to study Parkinson's disease
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The tif4631 null mutant is viable; the null mutant of paralog tif4632 is viable; the tif4631 tif4632 double mutant is inviable.
676 total interactions for 456 unique genes
Physical Interactions
- Affinity Capture-MS: 448
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 70
- Biochemical Activity: 1
- Co-crystal Structure: 2
- Co-fractionation: 1
- Co-localization: 2
- Co-purification: 3
- Far Western: 1
- FRET: 1
- Protein-RNA: 1
- Proximity Label-MS: 3
- Reconstituted Complex: 49
- Two-hybrid: 3
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 6
- Negative Genetic: 19
- Phenotypic Suppression: 2
- Positive Genetic: 25
- Synthetic Growth Defect: 16
- Synthetic Lethality: 8
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 8
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-14
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.