SIP4 / YJL089W Overview
- Standard Name
- SIP4 1 2
- Systematic Name
- YJL089W
- SGD ID
- SGD:S000003625
- Feature Type
- ORF , Verified
- Description
- C6 zinc cluster transcriptional activator; binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus 2 3 4
- Name Description
- SNF1-Interacting Protein 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 829
- Mol. Weight (Da)
- 95998.5
- Isoelectric Point
- 6.51
- Median Abundance (molecules/cell)
- 5733 +/- 4552
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SIP4 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding transcription factor that positively regulates gluconeogenesis, and negatively regulates invasive growth in response to glucose limitation; localizes to nucleus
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in negative regulation of invasive growth in response to glucose limitation (IMP)
- involved in positive regulation of gluconeogenesis (IPI, IMP, IGI)
- involved in positive regulation of transcription by RNA polymerase II (IGI, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
177 total interactions for 142 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 2
- Biochemical Activity: 6
- Reconstituted Complex: 1
- Two-hybrid: 10
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 5
- Dosage Rescue: 1
- Negative Genetic: 134
- Positive Genetic: 13
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SIP4 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. In the absence of glucose, Sip4p binds to carbon source response elements (CSREs) in the promoters of its target genes to activate transcription. Regulatory targets of Sip4p include genes involved in gluconeogenesis, such as FBP1, ICL1, and PCK1. Along with Sip4p, the transcription factors Cat8p and Rds2p also contribute to regulation of genes involved in gluconeogenesis. All three factors bind to target sites with the same consensus sequence, although each has a preference for particular variants of the sequence, and they have overlapping regulatory roles. Cat8p may have a more important role than Sip4p, since SIP4 is itself regulated by Cat8p and since the sip4 null mutant does not display defects in utilization of nonfermentable carbon sources. In the absence of glucose, SIP4 transcription is upregulated, and in addition, Sip4p is activated via phosphorylation, primarily by the Snf1p kinase.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.