DPB11 / YJL090C Overview
- Standard Name
- DPB11 1
- Systematic Name
- YJL090C
- SGD ID
- SGD:S000003626
- Feature Type
- ORF , Verified
- Description
- DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress 2 3 4 5 6 7 8 9 10
- Name Description
- DNA Polymerase B (II) 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 764
- Mol. Weight (Da)
- 87258.6
- Isoelectric Point
- 9.39
- Median Abundance (molecules/cell)
- 540 +/- 406
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DPB11 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of epsilon DNA polymerase complex and DNA replication preinitiation complex that activates protein kinases; involved in DNA replication, recombination and repair as well as mating type switching
View computational annotations
Molecular Function
- Manually Curated
- enables DNA polymerase binding (IDA)
- enables protein kinase activator activity (IDA)
Biological Process
- Manually Curated
- involved in DNA replication checkpoint signaling (IMP)
- involved in DNA replication initiation (IMP)
- involved in double-strand break repair via break-induced replication (IMP)
- involved in mating type switching (IMP)
- involved in mitotic G2 DNA damage checkpoint signaling (IGI)
- involved in positive regulation of DNA replication origin binding (IMP)
- involved in positive regulation of protein phosphorylation (IDA)
- involved in recombinational repair (IGI, IDA)
Cellular Component
- Manually Curated
- part of DNA replication preinitiation complex (IMP)
- located in nucleus (HDA)
- located in replication fork (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutant delays in S phase at the permissive temperature and arrests there at the restrictive temperature; conditional and repressible mutants display elevated rates of gross chromosomal rearrangements, chromosome loss and an increase in spontaneous DNA damage; conditional alleles display elevated heteroallelic recombination rates and reduced direct-repeat recombination; some conditional alleles display slow DSB repair kinetics and reduced efficiency of mating type switching; heterozygous diploid null is haploinsufficient; overexpression enhances invasive growth
Classical Genetics
- repressible
- null
- conditional
- cell cycle progression in S phase: arrested
- cell cycle progression in S phase: delayed
- chromosome/plasmid maintenance: decreased rate
- chromosome/plasmid maintenance: normal rate
- heat sensitivity: increased
- mating type switching: decreased
- mating type switching: normal
- mitotic recombination: abnormal
- resistance to chemicals: decreased
- null
- repressible
- reduction of function
- overexpression
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
851 total interactions for 590 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 44
- Biochemical Activity: 1
- Co-fractionation: 1
- Co-purification: 1
- Protein-peptide: 1
- Protein-RNA: 1
- Reconstituted Complex: 32
- Two-hybrid: 35
Genetic Interactions
- Dosage Growth Defect: 10
- Dosage Lethality: 1
- Dosage Rescue: 15
- Negative Genetic: 507
- Phenotypic Enhancement: 19
- Phenotypic Suppression: 12
- Positive Genetic: 102
- Synthetic Growth Defect: 21
- Synthetic Lethality: 19
- Synthetic Rescue: 12
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.