CDC45 / YLR103C Overview
- Standard Name
- CDC45 1
- Systematic Name
- YLR103C
- SGD ID
- SGD:S000004093
- Aliases
- SLD4 5
- Feature Type
- ORF , Verified
- Description
- DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes 2 3 4
- Name Description
- Cell Division Cycle 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CDC45/YLR103C is located on the right arm of chromosome XII between strong replication origin ARS1212.5 and uncharacterized gene LCL2; coding sequence is 1953 nucleotides long with 29 SNPs, 8 of which cause amino acid polymorphisms, and an in-frame 3-nucleotide insertion that codes an extra asparagine after residue D203 in strains D273-10B, SK1, Sigma1278b, and Y55
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cdc45p is 650 amino acids long, very low in abundance; phosphorylated on 6 residues
- Length (a.a.)
- 650
- Mol. Weight (Da)
- 74209.6
- Isoelectric Point
- 4.39
- Median Abundance (molecules/cell)
- 1632 +/- 714
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CDC45 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the CMG DNA helicase complex; involved in DNA replication initiation and double-strand break repair via break-induced replication
View computational annotations
Molecular Function
- Manually Curated
- enables chromatin binding (IDA)
- enables DNA replication origin binding (IDA)
- enables single-stranded DNA binding (IDA, IMP)
Biological Process
- Manually Curated
- involved in DNA replication initiation (IMP, IGI)
- involved in double-strand break repair via break-induced replication (IMP)
Cellular Component
- Manually Curated
- part of CMG complex (IDA)
- part of DNA replication preinitiation complex (IDA)
- located in nuclear replication fork (IDA)
- located in nucleus (IDA)
- part of replication fork protection complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; cold-sensitive, conditional mutant displays a first cycle arrest at the beginning of DNA replication under fully non-permissive conditions, and at medial nuclear division with a single large bud and nucleus in the bud neck under less restrictive conditions; homozygous diploid conditional mutant exhibits elevated rates of mitotic chromosome loss and recombination; repressible mutant experiences an S phase delay with increased chromosome rearrangements and chromosome loss; heterozygous diploid null mutant is haploinsufficient in rich media, and haploproficient in synthetic complete medium
Classical Genetics
- null
- cell cycle progression in S phase: delayed
- cell cycle progression through the G1/S phase transition: delayed
- chronological lifespan: abnormal
- growth in exponential phase: decreased rate
- hyperosmotic stress resistance: increased
- inviable
- replicative lifespan: increased
- resistance to chemicals: increased
- repressible
- conditional
- reduction of function
- unspecified
- null
- repressible
- reduction of function
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast CDC45 is homologous to human CDC45, and has been used to study mutations found in patients with Meier-Gorlin syndrome (MGS), a form of dwarfism caused by recessive mutations in genes encoding components of the pre-replication complex
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cdc45p interacts physically with proteins involved in DNA replication; CDC45 interacts genetically with genes involved in DNA repair
635 total interactions for 282 unique genes
Physical Interactions
- Affinity Capture-MS: 33
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 122
- Biochemical Activity: 1
- Co-fractionation: 9
- Co-localization: 7
- Co-purification: 17
- Protein-peptide: 2
- Reconstituted Complex: 63
- Two-hybrid: 10
Genetic Interactions
- Dosage Growth Defect: 5
- Dosage Lethality: 1
- Dosage Rescue: 6
- Negative Genetic: 205
- Phenotypic Enhancement: 10
- Phenotypic Suppression: 3
- Positive Genetic: 11
- Synthetic Growth Defect: 41
- Synthetic Lethality: 79
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CDC45 promoter is bound by Fkh1p, Mbp1p, Rap1p, and Swi6p; CDC45 transcription is regulated by Ixr1p; CDC45 transcription is upregulated by Yap5p in response to iron; Cdc45 protein activity is upregulated by Rad53p in response to hydroxyurea
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-09
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.