SWI6 / YLR182W Overview
- Standard Name
- SWI6 1
- Systematic Name
- YLR182W
- SGD ID
- SGD:S000004172
- Aliases
- PSL8 , SDS11 8
- Feature Type
- ORF , Verified
- Description
- Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators 2 3 4 5 6 7
- Name Description
- SWItching deficient 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 803
- Mol. Weight (Da)
- 90507.5
- Isoelectric Point
- 4.48
- Median Abundance (molecules/cell)
- 3241 +/- 1240
- Half-life (hr)
- 8.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SWI6 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription cofactor that activates transcription by RNA polymerase II during heat stress and during the G1/S mitotic transition; also regulates meiotic recombination; component of both the MBF and SBF complexes
View computational annotations
Molecular Function
- Manually Curated
- enables transcription coactivator activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in cellular response to heat (IMP)
- involved in G1/S transition of mitotic cell cycle (IMP)
- involved in positive regulation of reciprocal meiotic recombination (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IDA)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- part of MBF transcription complex (IDA)
- located in nucleus (IDA)
- part of SBF transcription complex (IDA, IMP, IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; mull mutant grows slowly, has large abnormally-shaped cells, cannot utilize various nitrogen sources, is impaired in fermentation, sensitive to caffeine, heat, cold, alcohols, metals, DNA-damaging agents, various antibiotics, antimalarial quinine, and oxidative stress; cell cycle progression is delayed at G1 and the G2/M transition; overexpression slows growth
Classical Genetics
- null
- cell cycle progression in G1 phase: increased duration
- cell shape: abnormal
- cell size: increased
- chemical compound accumulation: increased
- cold sensitivity: increased
- colony sectoring: increased
- fermentative metabolism: decreased rate
- fermentative metabolism: delayed
- hydrostatic pressure resistance: decreased
- killer toxin resistance: decreased
- metal resistance: decreased
- nuclear export: absent
- oxidative stress resistance: decreased
- protein/peptide accumulation: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: decreased
- viable
- overexpression
- reduction of function
- null
- cell cycle progression through the G2/M phase transition: delayed
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- heat sensitivity: increased
- innate thermotolerance: decreased
- innate thermotolerance: increased
- inviable
- lipid particle morphology: abnormal
- metal resistance: decreased
- mitotic recombination: decreased
- oxidative stress resistance: decreased
- petite-negative
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- toxin resistance: decreased
- toxin resistance: increased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1098 total interactions for 748 unique genes
Physical Interactions
- Affinity Capture-MS: 81
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 39
- Biochemical Activity: 10
- Co-crystal Structure: 1
- Co-localization: 1
- Co-purification: 1
- PCA: 6
- Protein-peptide: 1
- Proximity Label-MS: 2
- Reconstituted Complex: 10
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 6
- Dosage Rescue: 23
- Negative Genetic: 466
- Phenotypic Enhancement: 12
- Phenotypic Suppression: 25
- Positive Genetic: 235
- Synthetic Growth Defect: 83
- Synthetic Lethality: 69
- Synthetic Rescue: 10
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SWI6 encodes a transcription cofactor that regulates transcription of the G1/S gene cluster in the cell cycle as part of heterodimers formed with two different DNA-binding transcription factors, Swi4p and Mbp1p. With Swi4p, Swi6p forms the SBF (Swi4p/Swi6p cell-cycle box (SCB) binding factor) complex, whose targets include G1 cyclins (CLN1, CLN2, PCL1, PCL2) and the HO endonuclease. With Mbp1p, Swi6p forms the MBF (Mlu1 cell cycle box (MCB) Binding Factor) complex. MBF targets partially overlap with those of SBF, and include genes involved in DNA synthesis and DNA repair (for example, CDC21, CDC8, and CDC9, and G1 cyclins, as well as SWI4). SBF and MBF both repress G1/S genes during early G1 phase. Whi5p binding to the SBF complex in early G1 causes SBF to repress transcription. After phosphorylation of Whi5p by Cdc28p/Cln3p it dissociates from SBF, allowing SBF to activate transcription. Cdc28p/Cln3p also activates MBF-regulated promoters. Transcription of the CLN1 and CLN2 genes by SBF feeds back to further activate both SBF- and MBF-regulated promoters. Swi6p and SBF are also involved in chromatin reorganization at certain promoters such as CLN2 and HO via recruitment of the FACT complex. One target of MBF activation is Nrm1p, which binds to Swi6p at MBF target promoters and promotes the repressor activity of MBF. Increasing Cdc28p/Clb2p activity in late G1 phase promotes the repressor activity of both SBF and MBF. Phosphorylation of Swi6p by Cdc28p/Clb6p results in the accumulation of Swi6p in the cytoplasm in late G1, also helping to shut down transcription of the G1/S gene cluster as cells exit the G1 phase.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-12
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.