SEC14 / YMR079W Overview
- Standard Name
- SEC14 1
- Systematic Name
- YMR079W
- SGD ID
- SGD:S000004684
- Aliases
- PIT1 8 9
- Feature Type
- ORF , Verified
- Description
- Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication 2 3 4 6 7
- Name Description
- SECretory 5
- Paralog
- YKL091C 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SEC14/YMR079W is located on the right arm of chromosome XIII between CTF18 DNA replication protein and NAM7 ATP-dependent RNA helicase; coding sequence is 1071 nucleotides long with an intron at 10..165 and 8 SNPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Sec14p is 304 amino acids long, longer-lived, and high in abundance; contains 3 cellular retinaldehyde-binding protein signatures; carbamoylated on C17, acetylated on 4 lysines, ubiquitinylated on 6 lysines, phosphorylated on 10 residues
- Length (a.a.)
- 304
- Mol. Weight (Da)
- 34894.0
- Isoelectric Point
- 5.16
- Median Abundance (molecules/cell)
- 21229 +/- 4647
- Half-life (hr)
- 11.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SEC14 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytoplasmic phosphatidylcholine transporter and phosphatidylinositol carrier involved in phosphoslipid metabolism, Golgi-to-PM and Golgi-to-vacuole transport, and ascospore formation
View computational annotations
Molecular Function
- Manually Curated
- enables phosphatidylcholine transporter activity (IMP, IDA)
- enables phosphatidylinositol transfer activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in ascospore formation (IMP)
- involved in Golgi to plasma membrane protein transport (IMP)
- involved in Golgi to vacuole transport (IMP, IGI)
- involved in Golgi vesicle budding (IDA)
- involved in negative regulation of phosphatidylcholine biosynthetic process (IDA, IMP)
- involved in negative regulation of phosphatidylglycerol biosynthetic process (IMP)
- involved in phosphatidylinositol metabolic process (IMP, IGI)
- involved in phospholipid transport (IMP, IDA)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in cytosol (IDA, HDA)
- located in Golgi apparatus (IDA)
- located in Golgi membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; repressible alleles cause slow growth, abnormal mitochondrial morphology, and overproduction and excretion of inositol in the absence of inositol and choline (Opi- phenotype); conditional mutants have small nucleoli, decreased meiosis, absent sporulation, are auxotrophic for myo-inositol, accumulate Berkeley bodies, are defective in protein secretion and autophagy, and are sensitive to heat, antibiotics and antifungals; heterozygous diploid nulls are haploinsufficient
Classical Genetics
- null
- conditional
- autophagy: absent
- auxotrophy
- chemical compound accumulation: increased
- endomembrane system morphology: abnormal
- heat sensitivity: increased
- meiosis: decreased
- protein secretion: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- sporulation: absent
- subcellular morphology: abnormal
- repressible
- null
- repressible
- reduction of function
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast SEC14 is homologous to human SEC14L1, and has been used to study mutations found in patients with lymphovascular invasion related to breast cancer metastasis
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Sec14p interacts physically with proteins involved in proteolysis, SEC14 interacts genetically with genes involved in Golgi vesicle transport; the sec14 null mutant is inviable, the null mutant of paralog ykl091c is viable, the sec14 ykl091c double mutant has not been annotated for phenotype
414 total interactions for 274 unique genes
Physical Interactions
- Affinity Capture-MS: 19
- Affinity Capture-RNA: 5
- Co-localization: 1
- Co-purification: 1
- PCA: 2
- Proximity Label-MS: 3
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Rescue: 51
- Negative Genetic: 74
- Phenotypic Enhancement: 8
- Phenotypic Suppression: 17
- Positive Genetic: 19
- Synthetic Growth Defect: 116
- Synthetic Lethality: 47
- Synthetic Rescue: 41
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SEC14 promoter is bound by Yap6p in response to heat; SEC14 transcription is regulated by Sfp1p in response to stress and by Cad1p in response to hydrogen peroxide
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.