POR1 / YNL055C Overview
- Standard Name
- POR1 1
- Systematic Name
- YNL055C
- SGD ID
- SGD:S000005000
- Aliases
- VDAC 9 , YVDAC1 , OMP2
- Feature Type
- ORF , Verified
- Description
- Mitochondrial porin and phospholipid scramblase; voltage-dependent anion channel in the outer mitochondrial membrane required for maintenance of mitochondrial osmotic stability and membrane permeability; scramblase involved in mitochondrial transbilayer translocation of phospholipids; ubiquinone binding protein; couples glutathione pools of the intermembrane space and the cytosol; phosphorylated; protein abundance increases in response to DNA replication stress; orthologous to human VDAC1 and 2 2 3 4 5 6 7 8
- Name Description
- PORin 1
- Paralog
- POR2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 283
- Mol. Weight (Da)
- 30429.8
- Isoelectric Point
- 8.18
- Median Abundance (molecules/cell)
- 21873 +/- 16504
- Half-life (hr)
- 12.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all POR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Mitochondrial ubiquinone-binding voltage-gated monoatomic anion channel involved in apoptosis, redox homeostasis, DNA transport, and mitochondrion organization
View computational annotations
Molecular Function
- Manually Curated
- enables phospholipid scramblase activity (IDA)
- enables ubiquinone binding (IDA)
- enables voltage-gated monoatomic anion channel activity (IDA)
Biological Process
- Manually Curated
- involved in apoptotic process (IMP)
- involved in cell redox homeostasis (IMP)
- involved in DNA transport (IMP)
- involved in mitochondrion organization (IMP)
- involved in monoatomic ion transport (IDA)
- involved in phospholipid translocation (IMP)
- involved in positive regulation of protein import into nucleus (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- located in mitochondrial inner-outer membrane contact site (IPI)
- is active in mitochondrial outer membrane (IDA, IMP, HDA)
- located in mitochondrion (IPI, HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has reduced growth rate on nonfermentable carbon sources and respiratory growth is heat-sensitive; replicative lifespan of null mutant is extended more under caloric restriction than is wild-type lifespan; null mutant is sensitive to acid pH and oxidative stress, but resistant to rapamycin; in large-scale studies, null mutant shows reduced competitive fitness in a variety of media, small cell size, and altered resistance to various chemicals
Classical Genetics
- null
- apoptosis: decreased
- apoptosis: increased
- cell death: increased
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- heat sensitivity: increased
- mitochondrial morphology: abnormal
- protein activity: decreased
- protein/peptide distribution: abnormal
- redox state: abnormal
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased
- respiratory metabolism: decreased
- survival rate in stationary phase: decreased
- viability: decreased
- viability: increased
- overexpression
- unspecified
- gain of function
- null
- acid pH resistance: decreased
- biofilm formation: decreased
- cell size: decreased
- chemical compound accumulation: abnormal
- chronological lifespan: decreased
- competitive fitness: decreased
- haploinsufficient
- heat sensitivity: increased
- killer toxin resistance: decreased
- mitochondrial morphology: abnormal
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: increased
- toxin resistance: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased rate
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The por1 null mutant is viable; the null mutant of paralog por2 is viable; the por1 por2 double mutant displays a synthetic growth defect.
712 total interactions for 538 unique genes
Physical Interactions
- Affinity Capture-MS: 203
- Affinity Capture-RNA: 10
- Affinity Capture-Western: 38
- Co-fractionation: 4
- Co-purification: 2
- Cross-Linking-MS (XL-MS): 21
- PCA: 2
- Proximity Label-MS: 2
- Reconstituted Complex: 8
- Two-hybrid: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 2
- Negative Genetic: 328
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 7
- Positive Genetic: 46
- Synthetic Growth Defect: 20
- Synthetic Lethality: 6
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 14
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.