RAS2 / YNL098C Overview
- Standard Name
- RAS2 1
- Systematic Name
- YNL098C
- SGD ID
- SGD:S000005042
- Aliases
- CTN5 10 , CYR3 11 , GLC5 12 , TSL7
- Feature Type
- ORF , Verified
- Description
- GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; activated by increased levels of glycolysis intermediate fructose-1,6-bisphosphate; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication 2 3 4 5 6
- Name Description
- homologous to RAS proto-oncogene
- Paralog
- RAS1 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- RAS2 has a paralog, RAS1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 322
- Mol. Weight (Da)
- 34705.7
- Isoelectric Point
- 7.4
- Median Abundance (molecules/cell)
- 14938 +/- 3692
- Half-life (hr)
- 8.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RAS2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- GTPase involved in the regulation of filamentous growth and regulation of transcription in response to nutrient changes; primarily localized in the plasma membrane but has been detected in the ER membrane and mitochondria
View computational annotations
Molecular Function
- Manually Curated
- enables GTP binding (IDA)
- enables GTPase activity (IDA)
Biological Process
- Manually Curated
- involved in ascospore formation (IMP)
- involved in cellular response to glucose starvation (IMP)
- involved in cytoplasm to vacuole targeting by the Cvt pathway (IMP)
- involved in macroautophagy (IGI)
- involved in positive regulation of pseudohyphal growth (IMP)
- involved in positive regulation of transcription by galactose (IMP)
- involved in protein localization to bud neck (IGI)
- involved in regulation of cytoplasmic mRNA processing body assembly (IMP)
- involved in regulation of protein localization (IMP)
Cellular Component
- Manually Curated
- located in cell periphery (HDA)
- located in endoplasmic reticulum membrane (IDA)
- located in mitochondrion (IDA, HDA)
- located in nucleus (IDA)
- located in plasma membrane (HDA, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has a respiratory growth defect, displays enhanced efficiency of G1 arrest during nutrient depletion, increased accumulation of storage carbohydrates, decreased replicative but extended chronological lifespan, decreased filamentous and invasive growth, and an ability to sporulate on rich media; dominant hyperactive allele displays a loss of viability and decreased G1 arrest during nutrient depletion, fails to accumulate storage carbohydrates, has decreased replicative and chronological lifespan, a block in nitrogen starvation induced autophagy, reduced innate thermotolerance, enhanced pseudohyphal growth, and a reduction in sporulation; overexpression increases replicative lifespan and filamentous growth
Classical Genetics
- null
- budding index: decreased
- cell cycle progression in G1 phase: arrested
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: increased
- cold sensitivity: decreased
- colony appearance: abnormal
- desiccation resistance: decreased
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- hyperosmotic stress resistance: increased
- metal resistance: decreased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- protein transport: decreased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide modification: decreased
- protein/peptide modification: increased
- pseudohyphal growth: absent
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- RNA accumulation: increased
- sporulation: abnormal
- survival rate in stationary phase: increased
- vegetative growth: increased rate
- viable
- unspecified
- overexpression
- dominant negative
- activation
- autophagy: absent
- cell cycle progression in G1 phase: increased
- chemical compound accumulation: decreased
- chronological lifespan: decreased
- innate thermotolerance: decreased
- mitochondrial transport: decreased
- pexophagy: decreased
- protein activity: increased
- protein/peptide modification: absent
- pseudohyphal growth: increased
- replicative lifespan: decreased
- respiratory metabolism: decreased
- RNA accumulation: decreased
- sporulation efficiency: decreased
- sporulation: absent
- starvation resistance: decreased
- stress resistance: decreased
- utilization of carbon source: decreased
- viability: decreased
- reduction of function
- null
- biofilm formation: decreased
- chemical compound excretion: increased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- filamentous growth: decreased
- heat sensitivity: increased
- invasive growth: absent
- prion formation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- transposable element transposition: decreased
- vegetative growth: decreased
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast RAS2 is homologous to human KRAS, and has been used to study colorectal cancer
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The ras2 null mutant is viable; the null mutant of paralog ras1 is viable; the ras2 ras1 double mutant is inviable or displays a growth defect.
781 total interactions for 490 unique genes
Physical Interactions
- Affinity Capture-MS: 20
- Affinity Capture-RNA: 12
- Affinity Capture-Western: 12
- Biochemical Activity: 9
- Co-localization: 1
- Co-purification: 1
- PCA: 7
- Proximity Label-MS: 6
- Reconstituted Complex: 13
- Two-hybrid: 7
Genetic Interactions
- Dosage Growth Defect: 5
- Dosage Lethality: 7
- Dosage Rescue: 68
- Negative Genetic: 311
- Phenotypic Enhancement: 24
- Phenotypic Suppression: 72
- Positive Genetic: 71
- Synthetic Growth Defect: 33
- Synthetic Lethality: 31
- Synthetic Rescue: 71
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 15
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-03-04
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.