MSN1 / YOL116W Overview
- Standard Name
- MSN1 1
- Systematic Name
- YOL116W
- SGD ID
- SGD:S000005476
- Aliases
- FUP1 2 , HRB382 , MSS10 4 7 , PHD2 8
- Feature Type
- ORF , Verified
- Description
- Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress 1 2 3 4 5 6
- Name Description
- Multicopy suppressor of SNF1 mutation 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relative distribution to the nucleus increases upon DNA replication stress
- Length (a.a.)
- 382
- Mol. Weight (Da)
- 43057.0
- Isoelectric Point
- 6.69
- Median Abundance (molecules/cell)
- 612 +/- 341
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MSN1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding protein involved in positive regulation of transcription in response to different stresses; localizes to both nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables sequence-specific DNA binding (IDA)
Biological Process
- Manually Curated
- involved in cellular hyperosmotic salinity response (IGI, IMP)
- involved in cellular response to heat (IMP)
- involved in cellular response to methylglyoxal (IMP)
- involved in positive regulation of invasive growth in response to glucose limitation (IMP)
- involved in positive regulation of pseudohyphal growth (IMP)
- involved in positive regulation of starch catabolic process (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- auxotrophy
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- filamentous growth: decreased
- innate thermotolerance: decreased
- invasive growth: absent
- lipid particle morphology: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- stress resistance: decreased
- viable
- unspecified
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
357 total interactions for 319 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 6
- Co-localization: 1
- Two-hybrid: 3
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 12
- Negative Genetic: 252
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 8
- Positive Genetic: 62
- Synthetic Growth Defect: 2
- Synthetic Haploinsufficiency: 2
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MSN1 encodes a transcriptional activator without overall similarity to known transcription factor families. Msn1p binds DNA directly and localizes to the nucleus, and a LexA-Msn1p fusion functions as a transcriptional activator. MSN1 was discovered, and named, in several different genetic screens that reveal its various roles in filamentous growth, starch metabolism, carbon utilization, iron uptake, and stress responses. It was named MSN1 (Multicopy Suppressor of SNF) for suppression of the SUC2 (invertase) expression defect of a snf1 mutant; PHD2 (PseudoHyphal Determinant) for its ability to increase pseudohyphal growth when overexpressed; MSS10 (Multicopy Suppressor of STA10) for suppression of the glucoamylase production defect conferred by an allele of STA10 (FLO8) encoding a transcriptional activator; and FUP1 (Ferric Utilization Proficient) for its positive regulatory effect on iron uptake. In response to heat and osmotic stress, Msn1p activates transcription of GPD1, CTT1, and HSP12; it also activates GPD1 transcription in response to methylglyoxal stress. It is one of many transcriptional activators of the FLO11 gene, which encodes a cell wall adhesin involved in filamentous growth and cell-cell interactions.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.