NFI1 / YOR156C Overview
- Standard Name
- NFI1 1
- Systematic Name
- YOR156C
- SGD ID
- SGD:S000005682
- Aliases
- SIZ2 9
- Feature Type
- ORF , Verified
- Description
- SUMO E3 ligase; catalyzes sumoylation of Yku70p/80p and Sir4p promoting telomere anchoring to the nuclear envelope and regulating telomerase activity; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; sumoylates Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; role in telomere length maintenance 2 3 4 5 6 7 8
- Name Description
- Neck Filament Interacting 2
- Paralog
- SIZ1 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 726
- Mol. Weight (Da)
- 81177.1
- Isoelectric Point
- 7.28
- Median Abundance (molecules/cell)
- 755 +/- 201
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NFI1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- SUMO E3 ligase that sumoylates proteins involved in silencing (Sir3p, Sir4p, Yku70p and Yku80p), DNA repair (Rad59p, Rad52p and Rfa1p), the E3 ubiquitin ligase Rsp5p, histone variant Cse4p, septin Cdc3p and type II topoisomerase Top2; possesses dsDNA binding activity; chromatin associated
View computational annotations
Molecular Function
- Manually Curated
- enables double-stranded DNA binding (IDA, IMP)
- enables SUMO transferase activity (IMP, IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in chromosome segregation (IMP)
- involved in DNA double-strand break attachment to nuclear envelope (IGI)
- involved in protein sumoylation (IMP, IDA, IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
- null
- competitive fitness: decreased
- competitive fitness: increased
- heat sensitivity: decreased
- mitotic recombination: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- telomere length: increased
- transposable element transposition: decreased
- transposable element transposition: increased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The nfi1 null mutant is viable; the null mutant of paralog siz1 is viable; the nfi1 siz1 double mutant is inviable or displays a growth defect.
355 total interactions for 237 unique genes
Physical Interactions
- Affinity Capture-MS: 84
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 10
- Biochemical Activity: 18
- Co-localization: 1
- Co-purification: 1
- Protein-peptide: 1
- Reconstituted Complex: 12
- Two-hybrid: 40
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 3
- Negative Genetic: 60
- Phenotypic Enhancement: 26
- Phenotypic Suppression: 17
- Positive Genetic: 7
- Synthetic Growth Defect: 43
- Synthetic Lethality: 10
- Synthetic Rescue: 12
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.