BRO1 / YPL084W Overview
- Standard Name
- BRO1 1
- Systematic Name
- YPL084W
- SGD ID
- SGD:S000006005
- Aliases
- VPS31 8 9 , LPF2 , ASI6 , NPI3
- Feature Type
- ORF , Verified
- Description
- Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates protein sorting and deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes; required for ESCRT-dependent transport of ubiquitinated cargo to the vacuole; localizes to extracellular vesicles during heat conditioning with proposed function in thermotolerance; orthologous to mammalian ESCRT-associated protein ALIX (PDCD6IP) 2 3 4 5 6 7
- Name Description
- BCK1-like Resistance to Osmotic shock 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 844
- Mol. Weight (Da)
- 97253.6
- Isoelectric Point
- 5.05
- Median Abundance (molecules/cell)
- 4780 +/- 2168
- Half-life (hr)
- 9.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BRO1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein involved in ubiquitin-dependent catabolism via the multivesicular body sorting pathway; acts in targeting proteins to the endosome
View computational annotations
Molecular Function
- Manually Curated
- enables deubiquitinase activator activity (IDA)
- enables protein binding (IDA)
Biological Process
- Manually Curated
- involved in ATP export (IMP)
- involved in intralumenal vesicle formation (IGI, IMP)
- involved in late endosome to vacuole transport via multivesicular body sorting pathway (IMP)
- involved in mitochondria-associated ubiquitin-dependent protein catabolic process (IMP)
- involved in positive regulation of protein deubiquitination (IDA)
- involved in protein deubiquitination (IGI, IPI)
- involved in protein localization to endosome (IMP)
- involved in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway (IMP)
- involved in ubiquitin-dependent protein catabolic process (IMP)
- involved in vacuolar transport (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has a growth defect, is heat sensitive, and displays decreased competitive fitness on both fermentative and respiratory growth media; null mutant has a decreased ability to use raffinose as carbon source; null mutant has a reduced ability to target Doa4p to the endosome, an inability to sort MVB cargo protein to the vacuole and a strong vacuolar fragmentation defect; null mutant has decreased resistance to ionic stress, cadmium and manganese treatment, oxidative stress, and desiccation; null mutant is defective in regulating intracellular pH and displays decreased resistance to acid pH; null mutant has decreased telomere length, reduced innate thermotolerance, and a decreased ability to form biofilms; null mutant displays increased accumulation of cadmium, calcium and alanine and decreased accumulation of glycogen; homozygous diploid null mutant has a sporulation defect; heterozygous null mutant is haploinsufficient
Classical Genetics
- unspecified
- null
- acquired thermotolerance: decreased
- biofilm formation: decreased
- bud morphology: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- endocytosis: decreased
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- metal resistance: decreased
- metal resistance: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- utilization of carbon source: decreased
- viability: decreased
- reduction of function
- overexpression
- conditional
- null
- acid pH resistance: decreased
- biofilm formation: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: decreased
- chronological lifespan: increased
- colony appearance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- endoplasmic reticulum morphology: abnormal
- haploinsufficient
- heat sensitivity: increased
- innate thermotolerance: decreased
- metal resistance: decreased
- metal resistance: increased
- oxidative stress resistance: decreased
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- sporulation: absent
- stress resistance: decreased
- telomere length: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
379 total interactions for 319 unique genes
Physical Interactions
- Affinity Capture-MS: 23
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 15
- Co-localization: 3
- Co-purification: 1
- PCA: 6
- Proximity Label-MS: 2
- Reconstituted Complex: 11
- Two-hybrid: 11
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 2
- Negative Genetic: 183
- Phenotypic Enhancement: 10
- Phenotypic Suppression: 10
- Positive Genetic: 65
- Synthetic Growth Defect: 15
- Synthetic Lethality: 12
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 1
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2004-10-21
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.