BRO1 / YPL084W Overview


Standard Name
BRO1 1
Systematic Name
YPL084W
SGD ID
SGD:S000006005
Aliases
VPS31 5 6 , LPF2 , ASI6 , NPI3
Feature Type
ORF , Verified
Description
Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates protein sorting and deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes 2 3 4
Name Description
BCK1-like Resistance to Osmotic shock 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
844
Mol. Weight (Da)
97253.6
Isoelectric Point
5.05
Median Abundance (molecules/cell)
4780 +/- 2168
Half-life (hr)
9.7

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all BRO1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Protein involved in ubiquitin-dependent catabolism via the multivesicular body sorting pathway; acts in targeting proteins to the endosome

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant has a growth defect, is heat sensitive, and displays decreased competitive fitness on both fermentative and respiratory growth media; null mutant has a decreased ability to use raffinose as carbon source; null mutant has a reduced ability to target Doa4p to the endosome, an inability to sort MVB cargo protein to the vacuole and a strong vacuolar fragmentation defect; null mutant has decreased resistance to ionic stress, cadmium and manganese treatment, oxidative stress, and desiccation; null mutant is defective in regulating intracellular pH and displays decreased resistance to acid pH; null mutant has decreased telomere length, reduced innate thermotolerance, and a decreased ability to form biofilms; null mutant displays increased accumulation of cadmium, calcium and alanine and decreased accumulation of glycogen; homozygous diploid null mutant has a sporulation defect; heterozygous null mutant is haploinsufficient
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


379 total interactions for 319 unique genes

Physical Interactions

  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 15
  • Co-localization: 3
  • Co-purification: 1
  • PCA: 6
  • Proximity Label-MS: 2
  • Reconstituted Complex: 11
  • Two-hybrid: 11

Genetic Interactions

  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 183
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 10
  • Positive Genetic: 65
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 12
  • Synthetic Rescue: 5
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
1
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2004-10-21

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
48
Additional
61
Reviews
22

Resources