CLN2 / YPL256C Overview
- Standard Name
- CLN2 1
- Systematic Name
- YPL256C
- SGD ID
- SGD:S000006177
- Feature Type
- ORF , Verified
- Description
- G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1 1 2 3 4 5 6
- Name Description
- CycLiN 1
- Paralog
- CLN1 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CLN2 is located on the left arm of chromosome XVI toward the telomere between uncharacterized gene YPL257W and BBP1 spindle pole body duplication protein; coding sequence is 1638 nucleotides with 8 SNPs, 4 of which cause amino acid polymorphisms; CLN2 has paralog CLN1 from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cln2p is 545 amino acids long, low in abundance; contains cyclin domain; phosphorylated on 12 residues
- Length (a.a.)
- 545
- Mol. Weight (Da)
- 61685.2
- Isoelectric Point
- 5.62
- Median Abundance (molecules/cell)
- 1801 +/- 531
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CLN2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cyclin-dependent protein kinase (CDK) regulatory subunit involved in regulating passage through the cell cycle, as well as cell cycle re-entry after pheromone-induced arrest; localizes to both the cytoplasm and nucleus
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in re-entry into mitotic cell cycle after pheromone arrest (IGI)
- involved in regulation of protein phosphorylation (IDA)
- involved in traversing start control point of mitotic cell cycle (IGI, IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; dominant hyperactive alleles result in decreased critical cell size at START, decreased duration of the G1 phase and decreased pheromone-induced cell cycle arrest; null mutants display decreased invasive growth, starvation resistance, chronological lifespan, endocytosis and a decreased rate of nitrogen utilization; heterozygous diploid nulls are haploinsufficient
Classical Genetics
- null
- overexpression
- activation
- null
- chitin deposition: normal
- chronological lifespan: decreased
- colony appearance: abnormal
- competitive fitness: decreased
- endocytosis: decreased
- haploinsufficient
- resistance to chemicals: decreased
- starvation resistance: decreased
- transposable element transposition: increased
- utilization of nitrogen source: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast CLN2 is homologous to human CCNF, and has been used to study frontotemporal dementia and/or amyotrophic lateral sclerosis-5
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cln2p interacts physically with proteins involved in mitotic cell cycle regulation; CLN2 interacts genetically with genes involved in mitotic cell cycle regulation; the cln2 null mutant is viable; the null mutant of paralog cln1 is viable; the cln2 cln1 double mutant is viable; the cln1 cln2 cln3 triple mutant is inviable.
628 total interactions for 361 unique genes
Physical Interactions
- Affinity Capture-MS: 35
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 62
- Biochemical Activity: 3
- Co-localization: 1
- Co-purification: 1
- PCA: 1
- Protein-peptide: 1
- Protein-RNA: 1
- Reconstituted Complex: 12
- Two-hybrid: 5
Genetic Interactions
- Dosage Growth Defect: 9
- Dosage Lethality: 48
- Dosage Rescue: 36
- Negative Genetic: 279
- Phenotypic Enhancement: 14
- Phenotypic Suppression: 20
- Positive Genetic: 19
- Synthetic Growth Defect: 30
- Synthetic Lethality: 30
- Synthetic Rescue: 14
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 13
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-12-16
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.