UGA3 / YDL170W Overview


Standard Name
UGA3 1 2
Systematic Name
YDL170W
SGD ID
SGD:S000002329
Feature Type
ORF , Verified
Description
Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4 3 4 5 6
Name Description
Utilization of GAba 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type
Length (a.a.)
528
Mol. Weight (Da)
61219.4
Isoelectric Point
6.69
Median Abundance (molecules/cell)
1226 +/- 227

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all UGA3 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Promoter proximal region, sequence-specific DNA binding protein that recruits additional transcription factors to activate genes involved in the utilization of gamma-aminobutyrate (GABA)

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


91 total interactions for 85 unique genes

Physical Interactions

  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 5
  • Co-localization: 1
  • Two-hybrid: 1

Genetic Interactions

  • Negative Genetic: 63
  • Phenotypic Suppression: 1
  • Positive Genetic: 11
  • Synthetic Growth Defect: 8
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
UGA3 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Uga3p binds as a homodimer to UAS-GABA sequences in the promoters of target genes involved in uptake and utilization of the non-preferred nitrogen source gamma-aminobutyrate (GABA), such as UGA1, encoding GABA transaminase, and UGA4, encoding GABA permease. Transcriptional activation by Uga3p is promoted by GABA, probably because a conformational change upon GABA binding to Uga3p enhances its DNA-binding ability. Uga3p can activate transcription independently under artificial conditions (when overexpressed), but under physiological conditions it is one of several transcription factors involved in nitrogen source utilization (including Dal82p, Stp1p, and Stp2p) whose function depends on cooperation with the transcription factor Dal81p. The involvement of Dal81p in pathways for utilization of several different nitrogen sources allows the cell to integrate the disparate nutritional signals and to induce different pathways according to availability of each source. Binding of Uga3p to target promoters, and recruitment of the transcription coactivators SAGA and Mediator, is promoted by Dal81p. The mechanism by which Dal81p promotes Uga3p function probably involves physical interaction between the two transcription factors, since it does not require the DNA-binding domain of Dal81p.
Regulators
6
Targets
437
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
22
Additional
37
Reviews
8

Resources