HEM1 / YDR232W Overview
- Standard Name
- HEM1 1
- Systematic Name
- YDR232W
- SGD ID
- SGD:S000002640
- Aliases
- OLE3 1 19 , CYD1 20
- Feature Type
- ORF , Verified
- Description
- 5-aminolevulinate synthase; catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p; has a pyridoxal phosphate cofactor whose insertion is mediated by Mcx1p 1 2 3 4 5 6 7
- Name Description
- HEMe biosynthesis 1 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- HEM1/YDR232W is located near the middle of the right arm of chromosome IV between COX20 mitochondrial inner membrane protein and RTN1 reticulon protein; coding sequence is 1647 nucleotides long with 13 SNPs, two of which cause amino acid polymorphisms, and a 21-nucleotide deletion in Sigma1278b
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Hem1p is 548 amino acids long, shorter-lived, somewhat low in abundance; contains a pyridoxal-phosphate binding site; acetylated on K142, succinylated on K196, phosphorylated on T312
- Length (a.a.)
- 548
- Mol. Weight (Da)
- 59362.0
- Isoelectric Point
- 7.56
- Median Abundance (molecules/cell)
- 5028 +/- 2500
- Half-life (hr)
- 8.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HEM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- 5-aminolevulinate synthase involved in heme biosynthesis and positive regulation of organelle assembly; localizes to mitochondrial matrix
View computational annotations
Molecular Function
- Manually Curated
- enables 5-aminolevulinate synthase activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in heme biosynthetic process (IMP)
- involved in positive regulation of organelle assembly (IMP)
Cellular Component
- Manually Curated
- located in mitochondrial matrix (IDA)
- located in mitochondrion (HDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene is some backgrounds, non-essential in others; mutants are auxotrophic for cysteine and heme, have abnormal lipid particles, underproduce 5-aminolevulinic acid, are sensitive to various antibiotics, resistant to camptothecin, and cannot grow on glycerol or ethanol
Classical Genetics
- null
- repressible
- unspecified
- null
- repressible
- reduction of function
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast HEM1 is homologous to human ALAS1 and ALAS2, and has been used to study mutations found in patients with erythropoietic protoporphyria
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Hem1p interacts physically with proteins involved in RNA catabolism and translation regulation; HEM1 interacts genetically with genes involved in transcription
212 total interactions for 204 unique genes
Physical Interactions
- Affinity Capture-MS: 17
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 1
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 155
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 5
- Positive Genetic: 10
- Synthetic Growth Defect: 5
- Synthetic Lethality: 1
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-10-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.