MPT5 / YGL178W Overview
- Standard Name
- MPT5 1
- Systematic Name
- YGL178W
- SGD ID
- SGD:S000003146
- Aliases
- HTR1 , UTH4 , PUF5 12
- Feature Type
- ORF , Verified
- Description
- mRNA-binding protein of the PUF family; binds to specific mRNAs, often in the 3' UTR; has broad specificity and binds to more than 1000 mRNAs (16% of the transcriptome); recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity 1 2 3 4 5 7 8 9 10 11
- Name Description
- Multicopy suppressor of Pop Two 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mpt5p is 859 amino acids long, very short-lived, and low in abundance; contians 8 Pumilio RNA-binding repeats; phosphorylated on 29 residues
- Length (a.a.)
- 859
- Mol. Weight (Da)
- 95426.4
- Isoelectric Point
- 8.13
- Median Abundance (molecules/cell)
- 2316 +/- 859
- Half-life (hr)
- 2.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MPT5 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Messenger RNA (mRNA) binding involved in glutamate biosynthesis and methionine regeneration; localizes to cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables mRNA binding (HDA, IDA)
- enables protein-macromolecule adaptor activity (IPI)
Biological Process
- Manually Curated
- involved in intracellular mRNA localization (IMP)
- involved in negative regulation of translation (IDA)
- involved in negative regulation of translational initiation (IPI)
- involved in positive regulation of mRNA binding (IMP)
- involved in positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (IDA)
- involved in regulation of G1 to G0 transition (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null has elongated cells, decreased lifespan, increased heat sensitivity in W303, increased innate thermotolerance in S288C
Classical Genetics
- null
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Mpt5p interacts physically with proteins involved in transcription; MPT5 interacts genetically with genes involved in mitotic cell cycle
2305 total interactions for 1854 unique genes
Physical Interactions
- Affinity Capture-MS: 24
- Affinity Capture-RNA: 2105
- Affinity Capture-Western: 8
- Co-purification: 1
- PCA: 10
- Protein-RNA: 3
- Two-hybrid: 10
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 16
- Negative Genetic: 71
- Phenotypic Enhancement: 8
- Phenotypic Suppression: 5
- Positive Genetic: 8
- Synthetic Growth Defect: 17
- Synthetic Lethality: 7
- Synthetic Rescue: 11
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.