NAM7 / YMR080C Overview
- Standard Name
- NAM7 1
- Systematic Name
- YMR080C
- SGD ID
- SGD:S000004685
- Aliases
- SUP113 7 , IFS2 , MOF4 , UPF1 8 , SUT2 9
- Feature Type
- ORF , Verified
- Description
- ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress 2 3 4 5 6
- Name Description
- Nuclear Accommodation of Mitochondria 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 971
- Mol. Weight (Da)
- 109419.3
- Isoelectric Point
- 6.44
- Median Abundance (molecules/cell)
- 6133 +/- 1193
- Half-life (hr)
- 9.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NAM7 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATP-dependent RNA helicase and ATPase that is involved in non-sense mediated mRNA decay, translation termination, protein ubiquitination; localizes to the polyribosomes
View computational annotations
Molecular Function
- Manually Curated
- enables ATP hydrolysis activity (IDA)
- enables mRNA binding (HDA)
- enables ribosomal small subunit binding (IDA)
- enables RNA helicase activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in DNA recombination (IMP)
- involved in intracellular mRNA localization (IMP)
- involved in nuclear-transcribed mRNA catabolic process (IMP)
- involved in nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay (IGI, IMP)
- involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay (IMP)
- involved in protein ubiquitination (IMP, IDA, IPI)
- involved in regulation of translational termination (IMP)
- involved in silent mating-type cassette heterochromatin formation (IGI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays abnormal localization of proteins and RNAs to P-bodies, increased resistance to copper, and decreased Ty element transposition; in large-scale studies null mutant shows increased resistance to a variety of chemicals, as well as increased sensitivity to some chemicals
Classical Genetics
- null
- chemical compound accumulation: increased
- metal resistance: decreased
- metal resistance: increased
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: increased
- RNA localization: abnormal
- telomere length: decreased
- toxin resistance: increased
- transposable element transposition: decreased
- reduction of function
- null
- acid pH resistance: decreased
- bud morphology: abnormal
- competitive fitness: decreased
- competitive fitness: increased
- haploinsufficient
- heat sensitivity: increased
- killer toxin resistance: increased
- metal resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- telomere length: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of nitrogen source: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1142 total interactions for 886 unique genes
Physical Interactions
- Affinity Capture-MS: 114
- Affinity Capture-RNA: 522
- Affinity Capture-Western: 33
- Co-fractionation: 1
- Co-localization: 2
- Co-purification: 1
- PCA: 39
- Protein-RNA: 1
- Proximity Label-MS: 3
- Reconstituted Complex: 8
- Two-hybrid: 34
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 6
- Negative Genetic: 172
- Phenotypic Enhancement: 29
- Phenotypic Suppression: 14
- Positive Genetic: 99
- Synthetic Growth Defect: 21
- Synthetic Lethality: 4
- Synthetic Rescue: 38
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.