ZWF1 / YNL241C Overview
- Standard Name
- ZWF1 1
- Systematic Name
- YNL241C
- SGD ID
- SGD:S000005185
- Aliases
- MET19 29 , POS10
- Feature Type
- ORF , Verified
- Description
- Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adaptation to oxidative stress; negative regulator of nonselective autophagy, independent of Stb5p; protein abundance increases in response to DNA replication stress; homolog of human G6PD which is deficient in patients with hemolytic anemia; human G6PD can complement yeast zwf1 null mutant 1 2 3 4 5 6
- Name Description
- ZWischenFerment 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Zwf1p is 505 amino acids long, long-lived, moderate in abundance; acetylated on 5 lysines, phosphorylated on 13 residues, succinylated on K62, sumoylated on K302, ubiquitinylated on 3 lysines; protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 505
- Mol. Weight (Da)
- 57515.0
- Isoelectric Point
- 6.22
- Median Abundance (molecules/cell)
- 13982 +/- 5648
- Half-life (hr)
- 16.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ZWF1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytoplasmic glucose-6-phosphate dehydrogenase involved in the pentose-phosphate shunt, NADPH regeneration and response to hydrogen peroxide
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in NADPH regeneration (IMP)
- involved in pentose-phosphate shunt, oxidative branch (IMP)
- involved in response to hydrogen peroxide (IMP)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in S288C and W303, essential gene in Sigma1278b; null mutant is auxotrophic for methionine, displays abnormal vacuolar morphology, reduced competitive fitness, and decreased resistance to both pH and oxidative stresses; null displays increased resistance to DNA-damaging agents and antivirals; heterozygous null is haploinsufficient
Classical Genetics
- null
- autophagy: increased
- auxotrophy
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- growth in exponential phase: decreased rate
- growth in exponential phase: normal rate
- metal resistance: decreased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- protein activity: increased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: decreased
- stress resistance: increased
- utilization of carbon source: decreased
- vegetative growth: decreased
- vegetative growth: decreased rate
- vegetative growth: normal
- overexpression
- null
- acid pH resistance: decreased
- alkaline pH resistance: decreased
- competitive fitness: decreased
- haploinsufficient
- inviable
- oxidative stress resistance: decreased
- protein activity: increased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- silencing: decreased
- utilization of nitrogen source: absent
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast ZWF1 is homologous to human G6PD, and has been used to study congenital nonspherocytic hemolytic anemia
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Zwf1p interacts physically with proteins involved in protein catabolism and protein folding; ZWF1 interacts genetically with genes involved in chemical response and transcription
289 total interactions for 257 unique genes
Physical Interactions
- Affinity Capture-MS: 16
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 1
- Co-fractionation: 1
- Co-localization: 1
- Co-purification: 1
- Proximity Label-MS: 2
Genetic Interactions
- Dosage Rescue: 3
- Negative Genetic: 193
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 4
- Positive Genetic: 22
- Synthetic Growth Defect: 16
- Synthetic Lethality: 16
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 17
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-01-13
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.