MYO2 / YOR326W Overview
- Standard Name
- MYO2 5
- Systematic Name
- YOR326W
- SGD ID
- SGD:S000005853
- Aliases
- CDC66 35
- Feature Type
- ORF , Verified
- Description
- Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, lipid droplets, and the mitotic spindle; effector of Ypt11p, a rab-type small GTPase; MYO2 has a paralog, MYO4, that arose from the whole genome duplication 1 3 4 6
- Name Description
- MYOsin 2
- Paralog
- MYO4 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- MYO2 has a paralog, MYO4, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1574
- Mol. Weight (Da)
- 180661.9
- Isoelectric Point
- 6.24
- Median Abundance (molecules/cell)
- 6007 +/- 2768
- Half-life (hr)
- 9.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MYO2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Microfilament motor protein that binds actin and calmodulin; involved in vesicle transport along actin filaments, vesicle docking involved in exocytosis, and membrane addition at the site of cytokinesis; also involved in organelle inheritance, apical bud growth, polarized cell growth, and establishment of mitotic spindle orientation; subunit of the Myo2p-Vac17p-Vac8p transport complex; localizes to sites of polarized growth, incipient bud sites, vesicles, filamentous actin, and actin filament bundles
View computational annotations
Molecular Function
- Manually Curated
- enables actin filament binding (IDA)
- enables calmodulin binding (IDA)
- enables microfilament motor activity (IDA)
- enables protein binding (IPI)
- enables small GTPase binding (IPI)
Biological Process
- Manually Curated
- involved in establishment of mitotic spindle orientation (IPI, IMP)
- involved in Golgi inheritance (IMP)
- involved in intracellular distribution of mitochondria (IMP, IGI)
- involved in membrane addition at site of cytokinesis (IGI, IMP)
- involved in mitochondrion inheritance (IMP, IGI)
- involved in peroxisome inheritance (IMP, IPI)
- involved in vacuole inheritance (IGI, IPI, IMP)
- involved in vesicle docking involved in exocytosis (IGI)
- involved in vesicle transport along actin filament (IMP)
- involved in vesicle-mediated transport (IMP)
Cellular Component
- Manually Curated
- located in actin filament bundle (IMP)
- located in cellular bud neck (IDA)
- located in cellular bud tip (IDA)
- part of filamentous actin (IDA)
- located in fungal-type vacuole membrane (IDA)
- located in incipient cellular bud site (IDA)
- located in mating projection tip (IDA, HDA)
- part of meiotic nuclear membrane microtubule tethering complex (IMP)
- part of Myo2p-Vac17p-Vac8p transport complex (IPI)
- located in transport vesicle (IDA)
- located in vesicle (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional (ts) mutations result in cell cycle arrest and blocked cytokinesis; depletion leads to opi- phenotype (overproduction and excretion of inositol in the absence of inositol and choline); overexpression causes cell cycle arrest
Classical Genetics
- conditional
- null
- reduction of function
- null
- overexpression
- repressible
- reduction of function
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The myo2 null mutant is inviable; the null mutant of paralog myo4 is viable; the myo2 myo4 double mutant displays a synthetic growth defect.
1227 total interactions for 706 unique genes
Physical Interactions
- Affinity Capture-MS: 125
- Affinity Capture-RNA: 10
- Affinity Capture-Western: 49
- Biochemical Activity: 1
- Co-crystal Structure: 7
- Co-fractionation: 3
- Co-localization: 2
- Far Western: 1
- FRET: 1
- PCA: 18
- Protein-peptide: 2
- Proximity Label-MS: 1
- Reconstituted Complex: 32
- Two-hybrid: 64
Genetic Interactions
- Dosage Growth Defect: 8
- Dosage Lethality: 6
- Dosage Rescue: 30
- Negative Genetic: 652
- Phenotypic Enhancement: 8
- Phenotypic Suppression: 2
- Positive Genetic: 94
- Synthetic Growth Defect: 27
- Synthetic Lethality: 74
- Synthetic Rescue: 10
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-10-30
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.