Reference: Singh H and Dumas LB (1984) A DNA primase that copurifies with the major DNA polymerase from the yeast Saccharomyces cerevisiae. J Biol Chem 259(12):7936-40

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Abstract


Biochemical fractionation of the yeast Saccharomyces cerevisiae has revealed a novel DNA primase activity that copurifies with the major DNA polymerase activity. In the presence of RNA precursors and single-stranded DNA (poly(dT), M13), the DNA primase synthesizes discrete length oligoribonucleotides (apparent length, 8-12 nucleotides) as well as longer RNA chains that appear to be multiples of a modal length of 11-12 nucleotides. When DNA precursors are also present, the oligoribonucleotides are utilized by the accompanying DNA polymerase as primers for DNA synthesis. Copurification of these two enzymatic activities suggests their association in a physical complex which may function in the synthesis of Okazaki fragments at chromosomal replication forks.

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Journal Article | Research Support, U.S. Gov't, P.H.S.
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Singh H, Dumas LB
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