GAL83 / YER027C Overview
- Standard Name
- GAL83 1
- Systematic Name
- YER027C
- SGD ID
- SGD:S000000829
- Aliases
- SPM1
- Feature Type
- ORF , Verified
- Description
- One of three alternate beta-subunits of the Snf1 kinase complex; allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; necessary and sufficient for phosphorylation of the Mig2p transcription factor in response to alkaline stress; functionally redundant with SIP1 and SIP2 for the phosphorylation of Mig1p in response to glucose deprivation; contains a glycogen-binding domain 2 3 4 5
- Name Description
- GALactose metabolism 1
- Paralog
- SIP2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Gal83p is 417 amino acids long, low in abundance, shorter-lived; ubiquitinylated on K287, phosphorylated on 11 residues
- Length (a.a.)
- 417
- Mol. Weight (Da)
- 46619.7
- Isoelectric Point
- 4.47
- Median Abundance (molecules/cell)
- 3514 +/- 1035
- Half-life (hr)
- 7.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GAL83 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- AMP-activated protein kinase involved in cell adhesion, positive regulation of pseudohyphal growth and signal transduction; subunit of nucleotide-activated protein kinase complex; localizes to cytoplasm, nuclear envelope lumen and nucleus
View computational annotations
Molecular Function
- Manually Curated
- contributes to AMP-activated protein kinase activity (IMP, IGI)
Biological Process
- Manually Curated
- involved in cell adhesion (IMP)
- involved in invasive growth in response to glucose limitation (IMP)
- involved in regulation of protein-containing complex assembly (IGI)
- involved in signal transduction (IGI)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in nuclear envelope lumen (IDA)
- part of nucleotide-activated protein kinase complex (IPI, IDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null grows slowly, is defective in starvation-induced endocytosis, shows small defect in vacuolar fragmentation, decreased invasive growth, decreased chronological lifespan, cannot use raffinose as carbon source, is sensitive to zymolase, oleic acid, neomycin; overexpression interferes with fermentative metabolism, decreases heat sensitivity
Classical Genetics
- unspecified
- null
- overexpression
- reduction of function
- null
- auxotrophy
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased
- chronological lifespan: decreased
- colony appearance: abnormal
- competitive fitness: decreased
- competitive fitness: increased
- endocytosis: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Gal83p interacts physically with proteins involved in carbohydrate metabolism, GAL83 interacts genetically with genes involved in transcription; the gal83 null mutant is viable, the null mutant of paralog sip2 is viable, the gal83 sip2 double mutant is inviable or displays a growth defect
170 total interactions for 88 unique genes
Physical Interactions
- Affinity Capture-MS: 39
- Affinity Capture-Western: 5
- Biochemical Activity: 7
- Co-purification: 1
- Reconstituted Complex: 6
- Two-hybrid: 12
Genetic Interactions
- Dosage Growth Defect: 5
- Dosage Lethality: 2
- Dosage Rescue: 4
- Negative Genetic: 43
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 1
- Positive Genetic: 7
- Synthetic Growth Defect: 18
- Synthetic Lethality: 2
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-03-27
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.