MSW1 / YDR268W Overview
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Msw1p is 379 amino acids long, of average half-life, low in abundance; contains 4 tryptophan-tRNA ligase domains; acetylated and succinylated on K212
- Length (a.a.)
- 379
- Mol. Weight (Da)
- 43023.9
- Isoelectric Point
- 9.63
- Median Abundance (molecules/cell)
- 2482 +/- 1674
- Half-life (hr)
- 9.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MSW1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Mitochondrial tryptophanyl-tRNA synthetase that performs tryptophanyl-tRNA aminoacylation by coupling tryptophan to tryptophanyl-tRNA
View computational annotations
Molecular Function
- Manually Curated
- enables tryptophan-tRNA ligase activity (ISA, IMP)
Biological Process
- Manually Curated
- involved in mitochondrial tryptophanyl-tRNA aminoacylation (IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (IMP, HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- MSW1/YDR68W is a non-essential gene; null mutants are viable but exhibit a range of phenotypic changes including increased mitochondrial rho- mutation frequency, increased replicative lifespan, absent respiratory growth, decreased acid pH resistance, decreased chronological lifespan, decreased competitive fitness, decreased filamentous growth, increased heat sensitivity, decreased hyperosmotic stress resistance, absent invasive growth, abnormal lipid particle morphology, absent or decreased mitochondrial genome maintenance, increased oxidative stress resistance, decreased UV resistance, abnormal vacuolar morphology, decreased vegetative growth rate, absent or decreased utilization of carbon sources, and decreased respiratory growth rate. Reduction of function mutants exhibit absent or decreased respiratory growth rate. Overexpression of MSW1 results in decreased filamentous growth and absent invasive growth.
Classical Genetics
- null
- acid pH resistance: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chronological lifespan: decreased
- competitive fitness: decreased
- filamentous growth: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- invasive growth: absent
- lipid particle morphology: abnormal
- mitochondrial genome maintenance: absent
- mitochondrial genome maintenance: decreased
- oxidative stress resistance: increased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased
- respiratory growth: decreased rate
- stress resistance: increased
- utilization of carbon source: absent
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- UV resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast MSW1 is homologous to human WARS2, and has been used to study mutations found in patients with various presentations of mitochondrial disease, including growth retardation, progressive leukoencephalopathy, spastic paraparesis, tremor, ataxia, and infantile parkinsonism
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Msw1p interacts physically with proteins involved in mitochondrial translation; MSW1 interacts genetically with genes involved in transcription regulation and organelle organization
26 total interactions for 23 unique genes
Physical Interactions
- Affinity Capture-MS: 2
- Affinity Capture-RNA: 7
- Proximity Label-MS: 5
Genetic Interactions
- Dosage Growth Defect: 1
- Negative Genetic: 4
- Phenotypic Suppression: 1
- Synthetic Growth Defect: 4
- Synthetic Lethality: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MSW1 transcription is upregulated by Znf1p during xylose fermentation
- Regulators
- 1
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2008-07-14
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.