SGF73 / YGL066W Overview
- Standard Name
- SGF73 1
- Systematic Name
- YGL066W
- SGD ID
- SGD:S000003034
- Aliases
- SCA7 5
- Feature Type
- ORF , Verified
- Description
- Subunit of DUBm module of SAGA and SLIK; has roles in anchoring deubiquitination module (DUBm) into SAGA and SLIK complexes, maintaining organization and ubiquitin-binding conformation of Ubp8p, thereby contributing to overall DUBm activity; involved in preinitiation complex assembly at promoters; relocalizes to cytosol under hypoxia; human homolog ATXN7 implicated in spinocerebellar ataxia, and can complement yeast null mutant 1 2 3 4 5 6 7 8 9
- Name Description
- SaGa associated Factor, 73 kDa 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Sgf73p is 657 amino acids long, short-lived, low in abundance; contains disordered regions throughout; ubiquitinylated on K12, acetylated on 10 lysines, phosphorylated on 38 residues; subunit of DUBm module of SAGA and SLIK; relocalizes to cytosol under hypoxia
- Length (a.a.)
- 657
- Mol. Weight (Da)
- 72890.1
- Isoelectric Point
- 9.35
- Median Abundance (molecules/cell)
- 2419 +/- 811
- Half-life (hr)
- 4.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SGF73 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the DUBm deubiquitinating module of the SAGA and SLIK histone acetyltransferase complexes; involved in regulation of RNA polymerase II transcription preinitiation complex assembly, and nuclear mRNA export
View computational annotations
Molecular Function
- Manually Curated
- enables enzyme activator activity (IDA, IMP)
- contributes to histone acetyltransferase activity (IMP)
- enables structural molecule activity (IGI, IMP)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in chromatin organization (IMP)
- involved in mRNA export from nucleus (IMP)
- involved in positive regulation of RNA polymerase II transcription preinitiation complex assembly (IMP)
- involved in protein-containing complex assembly (IMP)
- involved in regulation of nucleocytoplasmic transport (IDA)
- involved in regulation of protein localization to chromatin (IDA)
Cellular Component
- Manually Curated
- located in cytosol (IDA)
- part of DUBm complex (IDA)
- located in nucleus (IDA)
- part of SAGA complex (IPI, IDA)
- part of SLIK (SAGA-like) complex (IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has a shortened chronological lifespan, but has a larger extension of replicative lifespan by caloric restriction than does wild type; null mutation or overexpression cause sensitivity to HU; null mutant displays slow growth on nonfermentable carbon sources; in large-scale studies, null mutant has reduced competitive fitness in several different media and displays chromosome instability, G2 or M phase delay in the mitotic cell cycle, abnormal meiosis, defective endocytosis, and altered transposition rates of different transposable elements; overexpression in the Sigma1278b background confers increased filamentous growth; diploid homozygous null mutant requires myo-inositol, accumulates less glycogen, and is sensitive to desiccation; null mutant displays altered resistance to a variety of chemicals
Classical Genetics
- null
- auxotrophy
- bud morphology: abnormal
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chronological lifespan: decreased
- colony sectoring: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- metal resistance: decreased
- metal resistance: increased
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- size of nucleolus: decreased
- sporulation efficiency: decreased
- sporulation: absent
- stress resistance: increased
- toxin resistance: decreased
- transposable element transposition: decreased
- transposable element transposition: increased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast SGF73 is homologous to human ATXN7, and has been used to study spinocerebellar ataxia type 7
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Sgf73p interacts physically with proteins involved in transcription; SGF73 interacts genetically with genes involved in transcription
1280 total interactions for 721 unique genes
Physical Interactions
- Affinity Capture-MS: 256
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 41
- Biochemical Activity: 3
- Co-localization: 1
- Co-purification: 41
- Proximity Label-MS: 1
- Reconstituted Complex: 6
- Two-hybrid: 5
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 684
- Phenotypic Suppression: 10
- Positive Genetic: 141
- Synthetic Growth Defect: 63
- Synthetic Lethality: 10
- Synthetic Rescue: 11
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Sgf73p is a subunit of the SAGA and SLIK chromatin modifying histone acetyltransferase complexes that acetylate histones and regulate gene expression. It plays a role in anchoring a deubiquitination module into SAGA and SLIK complexes. Sgf73p is involved in assembling the preinitiation complex at promoters. Sgf73p leaves the nucleus and localizes to the cytosol in response to hypoxia.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-08
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.