A role for the Mre11–Rad50–Xrs2 complex in gene expression and chromosome organization

Dataset: A role for the Mre11–Rad50–Xrs2 complex in gene expression and chromosome organization

External ID: GSE148170
Reference: Forey R, et al. (2021)
Channels: 1
Conditions: 29
Description: Mre11–Rad50–Xrs2 (MRX) is a highly conserved complex with key roles in various aspects of DNA repair. Here, we report a new function for MRX in limiting transcription in budding yeast. We show that MRX interacts physically and colocalizes on chromatin with the transcriptional co-regulator Mediator. It restricts transcription of coding and noncoding DNA independently by a mechanism that does not require the nuclease activity of Mre11. MRX is required to tether the active GAL locus to the nuclear pore complex (NPC) and also promotes large-scale gene–NPC interactions. Moreover, MRX-mediated chromatin anchoring to the NPC contributes to chromosome folding and helps to control gene expression. Together, these findings indicate that MRX has a role in transcription and chromosome organization that is distinct from its known function in DNA repair.
Categories: physical interaction

Conditions

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